CBX3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CBX3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

28

Rezensionen (1)

Kat-Nr. : 11650-2-AP

Synonyme

Modifier 2 protein, HP1Hs gamma, HP1 gamma, HECH

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Galerie der Validierungsdaten

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  WB analysis of mouse spleen using 11650-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11650-2-AP (CBX3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 11650-2-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11650-2-AP (CBX3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 11650-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11650-2-AP (CBX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 11650-2-AP

  IP result of anti-CBX3 (IP:11650-2-AP, 4ug; Detection:11650-2-AP 1:3000) with mouse spleen tissue lysate 1560 ug.

• 

  IHC staining of human colon cancer using 11650-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11650-2-AP (CBX3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 11650-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11650-2-AP (CBX3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11650-2-AP

  Immunofluorescent analysis of HepG2 cells, using CBX3 antibody 11650-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, A375-Zellen
• Erfolgreiche IP: Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 23 publications below
• IHC: See 8 publications below
• IF: See 8 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Produktinformation

11650-2-AP bindet in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA CBX3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CBX3 fusion protein Ag2251
• Vollständiger Name: chromobox homolog 3 (HP1 gamma homolog, Drosophila)
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC000954
• Gene symbol: CBX3
• Gene ID (NCBI): 11335
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The family of Heterochromatin protein 1 (HP1) proteins is a family of highly conserved heterochromatin-associated non-histone chromosomal proteins , which has important functions in nucleus. These functions include gene activation or repression, regulation of binding of cohesion complexes to centromere, sequestration of genes to nuclear periphery, and heterochromatin formation and propagation. Much evidence shows that HP1 proteins interact with numerous proteins including methylated histones, histone methyltransferases and so on. CBX3 is one of the paralogues of HP1 proteins

Publikationen

Species	Application	Title
mouse	IF	Nat Biotechnol; Retrospective analysis of enhancer activity and transcriptome history; Authors - Ruben Boers
human	WB	Cell; Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.; Authors - Xun Huang
human	WB,IF,IP	Adv Sci (Weinh); Targeting CBX3 with a Dual BET/PLK1 Inhibitor Enhances the Antitumor Efficacy of CDK4/6 Inhibitors in Prostate Cancer; Authors - Huaiyuan Liang ; KD Validated
mouse	WB,IP	J Exp Med; ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.; Authors - Longfei Ma
mouse	WB	Nat Commun; The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.; Authors - Guang Yang
human	WB,IHC,IF	J Cell Biol; Induction of alternative lengthening of telomeres-associated PML bodies by p53/p21 requires HP1 proteins.; Authors - Jiang Wei-Qin WQ ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

WEI (Verified Customer) (05-11-2022)

Strong bands for predicted size.

• Applications: Western Blot
• Cell Tissue Type: Mouse Heart