CBLB Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CBLB Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 12781-1-AP

Synonyme

RING-type E3 ubiquitin transferase CBL-B, RING finger protein 56, Nbla00127, EC:2.3.2.27, E3 ubiquitin-protein ligase CBL-B

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 12781-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12781-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12781-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12781-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12781-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12781-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 12781-1-AP

  IP result of anti-CBLB (IP:12781-1-AP, 4ug; Detection:12781-1-AP 1:600) with HEK-293 cells lysate 1800 ug.

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  IHC staining of mouse colon using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12781-1-AP (CBLB antibody) at dilution of 1:50 (under 10x lens)..

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  IHC staining of mouse colon using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12781-1-AP (CBLB antibody) at dilution of 1:50 (under 40x lens)..

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  IHC staining of human kidney using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 12781-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A431 using 12781-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CBLB antibody (12781-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 3 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

12781-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA CBLB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CBLB fusion protein Ag3491
• Vollständiger Name: Cas-Br-M (murine) ecotropic retroviral transforming sequence b
• Berechnetes Molekulargewicht: 982 aa, 109 kDa
• Beobachtetes Molekulargewicht: 110-130 kDa
• GenBank-Zugangsnummer: BC032851
• Gene symbol: CBLB
• Gene ID (NCBI): 868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CBLB(E3 ubiquitin-protein ligase CBL-B) is also named RNF56 and plays a necessary role in preventing the inappropriate activation of NF-B in response to lipopolysaccharide and polymicrobial sepsis. CBLB can be phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and various types of cell stimulation. It also has a crucial role in preventing chronic inflammation and autoimmunity. This protein has 3 isoforms produced by alternative splicing 109, 90, and 86 kDa, and observed molecular weight at 110-130 kDa.

Publikationen

Species	Application	Title
mouse	WB	J Exp Med; Cbl-b deficiency prevents functional but not phenotypic T cell anergy.; Authors - Trang T T Nguyen
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
rat	IHC	Sci Signal; Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn.; Authors - Zi-Yang Zhang
human	FC	J Immunol; A Multiple Sclerosis-Associated Variant of CBLB Links Genetic Risk with Type I IFN Function.; Authors - Klarissa Hanja Stürner
mouse	WB,IF	J Cell Mol Med; NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β-cells for protection.; Authors - Ze-Qing Pu
mouse	WB	Biomolecules; miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles.; Authors - Xin Yang