Caspase 2/p32/p18 Polyklonaler Antikörper

Caspase 2/p32/p18 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IP, RIP, ELISA, IF

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 10436-1-AP

Synonyme

CASP 2, CASP2, Caspase 2, Caspase 2/p18, Caspase 2/p32/p18, Caspase2, ICH 1L, ICH 1L/1S, ICH1, NEDD 2, NEDD2, Protease ICH 1

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Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 10436-1-AP

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (Caspase 2/p18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10436-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10436-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10436-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10436-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10436-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10436-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10436-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10436-1-AP

  IP result of anti-Caspase 2/p32/p18 (IP:10436-1-AP, 4ug; Detection:10436-1-AP 1:2000) with HeLa cells lysate 3000 ug.

• 

  IHC staining of human kidney using 10436-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10436-1-AP (Caspase 2/p18 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 10436-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10436-1-AP (Caspase 2/p18 antibody at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Staurosporin behandelte Jurkat-Zellen, HEK-293-Zellen, HeLa-Zellen, HL-60-Zellen, Jurkat-Zellen, Mauslebergewebe, Mausnierengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below
• ELISA: See 1 publications below
• RIP: See 1 publications below

Produktinformation

10436-1-AP bindet in WB, IHC, IP, RIP, ELISA, IF Caspase 2/p32/p18 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Caspase 2/p32/p18 fusion protein Ag0694
• Vollständiger Name: caspase 2, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 452 aa, 51 kDa
• Beobachtetes Molekulargewicht: 48-51 kDa, 32 kDa, 18kDa
• GenBank-Zugangsnummer: BC002427
• Gene symbol: Caspase 2
• Gene ID (NCBI): 835
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).

Publikationen

Species	Application	Title
human	WB, IF	Front Microbiol; Enterovirus 71 Induces INF2 Cleavage via Activated Caspase-2 in Infected RD Cells.; Authors - Bei Wang
		Front Pharmacol; PMID: 32210806; Authors - Wen Su
rabbit	RIP	Life Sci; Timolol induces necroptosis, apoptosis and senescence concentration-dependently in rabbit Limbal stem cells in vitro.; Authors - Guo-Jian Jiang
human	ELISA	Toxicol In Vitro; PMID: 32320760; Authors - Ting Yang
mouse	WB	Toxicon; Involvement of caspase in patulin-induced hepatotoxicity in vitro and in vivo.; Authors - Baigang Zhang
		Chem Biol Interact; Different concentrations of betaxolol switch cell fate between necroptosis, apoptosis, and senescence in human corneal stromal cells; Authors - Jing-Yu Sun