Caspase 12 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Caspase 12 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

91

Kat-Nr. : 55238-1-AP

Synonyme

CASP 12, CASP12, CASP12P1, Caspase 12, caspase 12 (gene/pseudogene), Caspase12, Inactive caspase 12

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 55238-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55238-1-AP (CASP12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of multi-cells using 55238-1-AP

  WB result of 55238-1-AP (CASP12 antibody) with various lysates.

• 

  WB analysis of Jurkat using 55238-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55238-1-AP (CASP12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55238-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55238-1-AP (CASP12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 55238-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 55238-1-AP (CASP12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 55238-1-AP

  IP Result of anti-Caspase 12 (IP:55238-1-AP, 4ug; Detection:55238-1-AP 1:1000) with HEK-293 cells lysate 2000ug.

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  IHC staining of human prostate hyperplasia using 55238-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 55238-1-AP (Caspase 12 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 55238-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 55238-1-AP (Caspase 12 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 55238-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55238-1-AP (Caspase 12 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 55238-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55238-1-AP (Caspase 12 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 55238-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 55238-1-AP (Caspase 12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Multizellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatahyperplasie-Gewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 88 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Produktinformation

55238-1-AP bindet in WB, IP, IHC, IF, ELISA Caspase 12 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, hamster, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: caspase 12 (gene/pseudogene)
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 36-42 kDa, 50 kDa
• GenBank-Zugangsnummer: NR_000035
• Gene symbol: CASP12
• Gene ID (NCBI): 120329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspase 12 is an enzyme known as a cysteine protease. It belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. It is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. Caspase-12 is located in the endoplasmic reticulum (ER). It is responsible for ER-stress-induced apoptosis, such as high calcium concentration, low oxygen and low glucose levels. Caspase-12 Antibody detects endogenous levels of full-length caspase-12 protein (50 kDa) and its cleaved product (40-44 kDa). This antibody does not cross-react with other caspases.

Publikationen

Species	Application	Title
human	WB	Autophagy; SARS-CoV-2 ORF3a induces RETREG1/FAM134B-dependent reticulophagy and triggers sequential ER stress and inflammatory responses during SARS-CoV-2 infection.; Authors - Xiaolin Zhang
human	WB	ACS Appl Mater Interfaces; Endoplasmic Reticulum-Targeted Aggregation-Induced Emission Luminogen for Synergetic Tumor Ablation with Glibenclamide; Authors - Yifan Wu
	WB	J Hazard Mater; Perinatal low-dose PBDE-47 exposure hampered thyroglobulin turnover and induced thyroid cell apoptosis by triggering ER stress and lysosomal destabilization contributing to thyroid toxicity in adult female rats.; Authors - Pei Li
human	WB,IF	Theranostics; Exosomes from mesenchymal stem cells modulate endoplasmic reticulum stress to protect against nucleus pulposus cell death and ameliorate intervertebral disc degeneration in vivo.; Authors - Zhiwei Liao
human	WB	Biomed Pharmacother; A novel synthetic curcumin derivative MHMM-41 induces ROS-mediated apoptosis and migration blocking of human lung cancer cells A549.; Authors - Guang-Zhou Zhou
human	WB	Oxid Med Cell Longev; Betulinic Acid Suppresses Breast Cancer Metastasis by Targeting GRP78-Mediated Glycolysis and ER Stress Apoptotic Pathway.; Authors - Yifeng Zheng

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