CAPN1 Monoklonaler Antikörper

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• KD/KO Validated

CAPN1 Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

4E6H1

Kat-Nr. : 67732-1-Ig

Synonyme

Calpain 1 catalytic subunit, Calpain 1 large subunit, Calpain mu type, CANP, CANP 1, CANP1, CANPL1, CAPN1, Micromolar calpain, muCANP, muCL

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Galerie der Validierungsdaten

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  WB analysis using 67732-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67732-1-Ig (CAPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67732-1-Ig

  WB result of CAPN1 antibody (67732-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAPN1 transfected HeLa cells.

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  WB analysis using 67732-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67732-1-Ig (CAPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human urothelial carcinoma using 67732-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67732-1-Ig (CAPN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 67732-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67732-1-Ig (CAPN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 67732-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67732-1-Ig (CAPN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 67732-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67732-1-Ig (CAPN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, Jurkat-Zellen, K562-Zellen, LNCaP-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IHC: humanes Urothelkarzinomgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

67732-1-Ig bindet in WB, IHC, IF, ELISA CAPN1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CAPN1 fusion protein Ag30522
• Vollständiger Name: calpain 1, (mu/I) large subunit
• Berechnetes Molekulargewicht: 704 aa, 82 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC008751
• Gene symbol: CAPN1
• Gene ID (NCBI): 823
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; Polysaccharides from Polygonatum cyrtonema Hua Reduce Depression-Like Behavior in Mice by Inhibiting Oxidative Stress-Calpain-1-NLRP3 Signaling Axis.; Authors - Fengming Shen
human	WB	Cell Rep; Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.; Authors - Stephanie Pei Tung Yiu
mouse	WB,IF	Neural Plast; Astragaloside IV Alleviates Brain Injury Induced by Hypoxia via the Calpain-1 Signaling Pathway; Authors - Yan Meng