CaMKII Beta Polyklonaler Antikörper

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• KD/KO Validated

CaMKII Beta Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 11533-1-AP

Synonyme

CaM kinase II subunit beta, CAM2, CaMK II subunit beta, CAMK2, CAMK2B, CAMKB, CaMKII beta

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Galerie der Validierungsdaten

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  WB analysis using 11533-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11533-1-AP (CaMKII beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11533-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11533-1-AP (CaMKII beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11533-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11533-1-AP (CaMKII beta antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 11533-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11533-1-AP (CaMKII beta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11533-1-AP

  IP result of anti-CaMKII Beta (IP:11533-1-AP, 4ug; Detection:11533-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 11533-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11533-1-AP (CaMKII beta antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 11533-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11533-1-AP (CaMKII beta antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 11533-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11533-1-AP (CaMKII beta antibody at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

11533-1-AP bindet in WB, IP, IF, IHC, ELISA CaMKII Beta und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CaMKII Beta fusion protein Ag2119
• Vollständiger Name: calcium/calmodulin-dependent protein kinase II beta
• Berechnetes Molekulargewicht: 503 aa, 56 kDa
• Beobachtetes Molekulargewicht: 50-70 kDa
• GenBank-Zugangsnummer: BC019070
• Gene symbol: CaMKII beta
• Gene ID (NCBI): 816
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CAMK2B(Calcium/calmodulin-dependent protein kinase type II subunit beta ), also named CAM2, CAMK2, and CAMKB, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. It is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2B is a member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It plays a distinct role in the induction of anergy in T lymphocytes, by differential regulation of IL10 and IL2 gene transcription suggesting MEF2A as a molecular target that can integrate different calcium signals(PMID:22578382). This protein has 8 isoforms produced by alternative splicing with a molecular weight between 50 kDa and 73 kDa. This antibody may have cross-reaction with CAMK2A/D/G due to the high homology.

Publikationen

Species	Application	Title
rat	WB	Aging (Albany NY); Gastrodin ameliorates learning and memory impairment in rats with vascular dementia by promoting autophagy flux via inhibition of the Ca2+/CaMKII signal pathway.; Authors - Ting-Ting Chen
mouse	WB	Sci Total Environ; Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional brain regions and dopaminergic neurons.; Authors - Huanliang Liu
human	WB,IHC	Front Oncol; Anti-Tumor Role of CAMK2B in Remodeling the Stromal Microenvironment and Inhibiting Proliferation in Papillary Renal Cell Carcinoma.; Authors - Qingan Jia ; KD Validated
human	IP	Front Cell Neurosci; Pin1 Is Regulated by CaMKII Activation in Glutamate-Induced Retinal Neuronal Regulated Necrosis.; Authors - Shuchao Wang
mouse	WB	PLoS One; Adaptive Gene Regulation in the Striatum of RGS9-Deficient Mice.; Authors - Kathy Busse
rat	WB,IF	Neural Plast; Ketamine Induces Lasting Antidepressant Effects by Modulating the NMDAR/CaMKII-Mediated Synaptic Plasticity of the Hippocampal Dentate Gyrus in Depressive Stroke Model.; Authors - Idriss Ali Abdoulaye