CAMK1D Polyklonaler Antikörper

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• KD/KO Validated

CAMK1D Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13613-1-AP

Synonyme

CaM K1, CaM KI delta, CaM kinase I delta, CaM kinase ID, CAMK1D, CaMKI delta, CaMKI like protein kinase, CaMKID, CKLiK

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 13613-1-AP

  WB result of CAMK1D antibody (13613-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAMK1D transfected HeLa cells.

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  WB analysis using 13613-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13613-1-AP (CAMK1D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 13613-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13613-1-AP (CAMK1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 13613-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13613-1-AP (CAMK1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 13613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 13613-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 13613-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe, HeLa-Zellen, Maus-Thymusgewebe, Ratten-Pankreasgewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

13613-1-AP bindet in WB, IHC, ELISA CAMK1D und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CAMK1D fusion protein Ag4517
• Vollständiger Name: calcium/calmodulin-dependent protein kinase ID
• Berechnetes Molekulargewicht: 357 aa, 40 kDa
• Beobachtetes Molekulargewicht: 38-42 kDa
• GenBank-Zugangsnummer: BC035745
• Gene symbol: CAMK1D
• Gene ID (NCBI): 57118
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.