Calretinin Polyklonaler Antikörper

Calretinin Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 12278-1-AP

Synonyme

29 kDa calbindin, CAB29, CAL2, CALB2, calbindin 2, Calretinin, CR

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Galerie der Validierungsdaten

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  WB analysis using 12278-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12278-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 12278-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 12278-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human ileum using 12278-1-AP

  human ileum tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12278-1-AP

  IP result of anti-Calretinin (IP:12278-1-AP, 4ug; Detection:12278-1-AP 1:10000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of human appendicitis using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human cerebellum using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human cerebellum using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, humanes Hirngewebe, humanes Ileumgewebe, humanes Kolongewebe, Maushirngewebe, Maus-Kolongewebe, Rattenhirngewebe, Ratten-Kolongewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Appendizitis-Gewebe, humanes Cerebellum-Gewebe, humanes Hirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 8 publications below

Produktinformation

12278-1-AP bindet in WB, IP, IF, IHC, ELISA Calretinin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Calretinin fusion protein Ag2924
• Vollständiger Name: calbindin 2
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC015484
• Gene symbol: Calretinin
• Gene ID (NCBI): 794
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.

Publikationen

Species	Application	Title
human	IF	Cell Rep; Mesothelial cells with mesenchymal features enhance peritoneal dissemination by forming a protumorigenic microenvironment; Authors - Atsuko Yonemura
mouse	IF	Front Cell Dev Biol; Stepwise Induction of Inner Ear Hair Cells From Mouse Embryonic Fibroblasts via Mesenchymal- to-Epithelial Transition and Formation of Otic Epithelial Cells.; Authors - Qiong Yang
human	IF	Stem Cells Int; Characterization of Primary Cilia Formation in Human ESC-Derived Retinal Organoids; Authors - Ke Ning
human	IHC	Front Mol Biosci; Tumor Microenvironment Heterogeneity-Based Score System Predicts Clinical Prognosis and Response to Immune Checkpoint Blockade in Multiple Colorectal Cancer Cohorts.; Authors - Hufei Wang
mouse	IHC	Endocrinology; Fetal Bisphenol-A Induced Changes in Murine Behavior and Brain Gene Expression Persist in Aged Offspring.; Authors - Zhihao Wang
mouse	IF	Invest Ophthalmol Vis Sci; Primary Cilia in Amacrine Cells in Retinal Development.; Authors - Ke Ning