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CADM3 Polyklonaler Antikörper

CADM3 Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC

Konjugation

Unkonjugiert

Kat-Nr. : 15660-1-AP

Synonyme

BIgR, Brain immunoglobulin receptor, CADM3, cell adhesion molecule 3, IGSF4B, Necl 1, NECL1, Nectin like protein 1, synCAM3, TSLC1 like protein 1, TSLL1

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of various lysates using CADM3 Polyclonal antibody (15660-1-AP)

    WB analysis using 15660-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15660-1-AP (CADM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CADM3 Polyclonal antibody (15660-1-AP)

    WB analysis of Jurkat using 15660-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15660-1-AP (CADM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using CADM3 Polyclonal antibody (15660-1-AP)

    WB analysis of SH-SY5Y using 15660-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15660-1-AP (CADM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using CADM3 Polyclonal antibody (15660-1-AP)

    FC experiment of SH-SY5Y using 15660-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug CADM3 antibody (15660-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaushirngewebe, Jurkat-Zellen, Maus-Cerebellum-Gewebe, Rattenhirngewebe, SH-SY5Y-Zellen
Erfolgreiche Detektion in FCSH-SY5Y-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Durchflusszytometrie (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

15660-1-AP bindet in WB, FC CADM3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CADM3 fusion protein Ag8081
Vollständiger Name cell adhesion molecule 3
Berechnetes Molekulargewicht 43 kDa
Beobachtetes Molekulargewicht 38-50 kDa
GenBank-ZugangsnummerBC033819
Gene symbol CADM3
Gene ID (NCBI) 57863
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CADM3, also known as IGSF4B or TSLL1, is a transmembrane protein that is involved in the cell-cell adhesion. CADM3 is mainly expressed in the nervous system and plays a role in the formation of synapses, axon bundles, myelinated axons and cerebellar morphogenesis (PMID: 18420026).

Protokolle

Produktspezifische Protokolle
WB protocol for CADM3 antibody 15660-1-APProtokoll herunterladen
FC protocol for CADM3 antibody 15660-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen