CA9 Polyklonaler Antikörper

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• KD/KO Validated

CA9 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 11443-1-AP

Synonyme

CA IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, carbonic anhydrase IX, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC associated antigen G250

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Galerie der Validierungsdaten

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  WB analysis of A549 using 11443-1-AP

  WB result of CA9 antibody (11443-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CA9 transfected A549 cells.

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  WB analysis of mouse brain using 11443-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11443-1-AP (CA IX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 11443-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11443-1-AP (CA9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11443-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11443-1-AP (CA9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11443-1-AP bindet in WB, IHC, ELISA CA9 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CA9 fusion protein Ag1963
• Vollständiger Name: carbonic anhydrase IX
• Berechnetes Molekulargewicht: 459 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC014950
• Gene symbol: CA9
• Gene ID (NCBI): 768
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CA9(Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia(PMID:18703501).It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emma (Verified Customer) (10-28-2022)

Western blot, 30 µg lysate per well. Glioblastoma stem-like cells were cultured as small speroids and treated with CoCl2 to stabilise HIF-1a, or as medium or XL speroids (no CoCl2 treatment). There were are a few bands visible on the blot, but CoCl2 induced a band at around 60 kDa, and XL speroids which have a hypoxic core also resulted in a heavy band at the same height. See also: https://www.nature.com/articles/s41389-022-00433-3#Sec18 Figure S5 and supplementary methods for WB details.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioblastoma stem-like cells

CA9 Antibody Western Blot validation (1:1000 dilution) in Glioblastoma stem-like cells (Cat no:11443-1-AP)