CA8 Polyklonaler Antikörper

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• KD/KO Validated

CA8 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 12391-1-AP

Synonyme

CA VIII, CA8, CALS, carbonic anhydrase VIII, CARP

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Galerie der Validierungsdaten

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  WB analysis using 12391-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12391-1-AP (CA8 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12391-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12391-1-AP (CA8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12391-1-AP

  IP result of anti-CA8 (IP:12391-1-AP, 4ug; Detection:12391-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 12391-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12391-1-AP (CA8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12391-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12391-1-AP (CA8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 12391-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12391-1-AP (CA8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 12391-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12391-1-AP (CA8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Produktinformation

12391-1-AP bindet in WB, IP, IF, IHC, ELISA CA8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CA8 fusion protein Ag3068
• Vollständiger Name: carbonic anhydrase VIII
• Berechnetes Molekulargewicht: 290 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC015531
• Gene symbol: CA8
• Gene ID (NCBI): 767
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The CA8 (CARP) gene encodes carbonic anhydrase VIII, which is part of a family of zinc metalloenzyme. CA8 has a central carbonic anhydrase motif, but it lacks carbonic anhydrase activity due to absence of catalytic zinc coordinating residues(PMID:2121526). CARP is a novel IP3R1-binding protein, and is expressed in Purkinje cells abundantly. CA8 is co-localized with IP3R1 in Purkinje cells and it binds to IP3R1, reducing the affinity of the receptor for its ligand, IP3(PMID:12611586). Defects in CA8 are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 3 (CMARQ3)(PMID:19461874).

Publikationen

Species	Application	Title
	IP	Nucleic Acids Res; Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing.; Authors - Ken Asada ; KD Validated
mouse	IF	J Am Soc Nephrol; Single-Cell RNA Sequencing Reveals Renal Endothelium Heterogeneity and Metabolic Adaptation to Water Deprivation.; Authors - Sébastien J Dumas
human	WB,IHC	Biomed Pharmacother; CA8 promotes RCC proliferation and migration though its expression level is lower in tumor compared to adjacent normal tissue.; Authors - Huai-Lu Ma ; KD Validated
rat	IHC	PLoS One; Mechanisms that determine the internal environment of the developing brain: a transcriptomic, functional and ultrastructural approach.; Authors - Liddelow Shane A SA
mouse	IHC	Am J Physiol Gastrointest Liver Physiol; Carbonic anhydrase 8 (CAR8) negatively regulates GLP-1 secretion from enteroendocrine cells in response to long-chain fatty acids.; Authors - Yuta Fujiwara
mouse	IHC	Neuron; Disruption of the ATXN1-CIC complex reveals the role of additional nuclear ATXN1 interactors in spinocerebellar ataxia type 1; Authors - Stephanie L Coffin