C4orf49 Polyklonaler Antikörper

C4orf49 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22569-1-AP

Synonyme

C4orf49, CESP-1, Corneal endothelium-specific protein 1, HUMMR, MGARP, OSAP, Ovary specific acidic protein, Protein MGARP

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Galerie der Validierungsdaten

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  WB analysis of A2780 using 22569-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 22569-1-AP (C4orf49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 22569-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 22569-1-AP (C4orf49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 22569-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22569-1-AP (C4orf49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 22569-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22569-1-AP (C4orf49 Antibody) at dilution of 1:50.

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  IHC staining of human ovary using 22569-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary slide using 22569-1-AP (C4orf49 Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 22569-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22569-1-AP (C4orf49 Antibody) at dilution of 1:50.

• 

  IHC staining of human ovary using 22569-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary slide using 22569-1-AP (C4orf49 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, A549-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Eierstockgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

22569-1-AP bindet in WB, IHC,ELISA C4orf49 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C4orf49 fusion protein Ag16552
• Vollständiger Name: chromosome 4 open reading frame 49
• Berechnetes Molekulargewicht: 240 aa, 25 kDa
• Beobachtetes Molekulargewicht: 33-36 kDa
• GenBank-Zugangsnummer: BC104174
• Gene symbol: C4orf49
• Gene ID (NCBI): 84709
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The protein MGARP, also named as C4ORF49, CESP1, HUMMR, OSAP and GS3582, plays a role in the trafficking of mitochondria along microtubules. It regulates the kinesin-mediated axonal transport of mitochondria to nerve terminals along microtubules during hypoxia. MGARP is localized to the mitochondria in cultured human CE, and its N-terminal seems to be processed after mitochondrial import. The MW of MGARP is about 33-36 kDa(PMID: 16565373).