C4 Alpha Chain/C4b/C4d Polyklonaler Antikörper

C4 Alpha Chain/C4b/C4d Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 22233-1-AP

Synonyme

Acidic complement C4, C4, C4A, C4A2, C4A3, C4A4, C4A6, C4S, CO4, Complement C4 A, CPAMD2, RG

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Galerie der Validierungsdaten

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  WB analysis using 22233-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22233-1-AP (C4 Alpha Chain/C4b/C4d antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of human blood using 22233-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 22233-1-AP (C4 alpha chain/C4b/C4d antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 22233-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22233-1-AP (C4 Alpha Chain/C4b/C4d antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 22233-1-AP

  IP Result of anti-C4 alpha chain/C4b/C4d (IP:22233-1-AP, 4ug; Detection:22233-1-AP 1:1000) with L02 cells lysate 2000ug.

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  IHC staining of human liver using 22233-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22233-1-AP (C4 alpha chain/C4b/C4d antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 22233-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22233-1-AP (C4 alpha chain/C4b/C4d antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, Mauslebergewebe
• Erfolgreiche IP: L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:4000-1:40000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

22233-1-AP bindet in WB, IP, IHC, ELISA C4 Alpha Chain/C4b/C4d und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C4 Alpha Chain/C4b/C4d fusion protein Ag17583
• Vollständiger Name: complement component 4A (Rodgers blood group)
• Berechnetes Molekulargewicht: 1744 aa, 193 kDa
• Beobachtetes Molekulargewicht: 80-95 kDa, 200 kDa
• GenBank-Zugangsnummer: BC063289
• Gene symbol: C4A
• Gene ID (NCBI): 720
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular wights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1017-1336aa of human C4-A protein can recognize C4 precursor, C4 alpha chain, C4b, and C4d.

Publikationen

Species	Application	Title
		Brain; Extracellular vesicle biomarkers for complement dysfunction in schizophrenia; Authors - Ting Xue
mouse	IHC	Cell Biochem Funct; In silico analysis and verification of critical genes related to vascular calcification in multiple diseases; Authors - Shicheng Li
human	WB	Immunobiology; Inefficient and abortive classical complement pathway activation by the calcium inositol hexakisphosphate component of the Echinococcus granulosus laminated layer.; Authors - Anabella A Barrios
human	WB	Transplant Direct; Circulating Donor Heart Exosome Profiling Enables Noninvasive Detection of Antibody-mediated Rejection.; Authors - Robert W Hu