Hikeshi Polyklonaler Antikörper

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• KD/KO Validated

Hikeshi Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 14808-1-AP

Synonyme

C11orf73, Hikeshi, HSPC138, HSPC179, HSPC248, L7RN6

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 14808-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14808-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 14808-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 14808-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14808-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14808-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 14808-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14808-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14808-1-AP

  IP Result of anti-Hikeshi (IP:14808-1-AP, 4ug; Detection:14808-1-AP 1:500) with mouse brain tissue lysate 2640ug.

• 

  IHC staining of human kidney using 14808-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14808-1-AP (Hikeshi antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14808-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14808-1-AP (Hikeshi antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, A375-Zellen, Maushirngewebe, PC-3-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

14808-1-AP bindet in WB, IP, IHC, ELISA Hikeshi und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Hikeshi fusion protein Ag6530
• Vollständiger Name: chromosome 11 open reading frame 73
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC001677
• Gene symbol: C11orf73
• Gene ID (NCBI): 51501
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
		Oncogene; HSP110 promotes colorectal cancer growth through STAT3 activation.; Authors - K Berthenet
human	WB	J Med Genet; Leukoencephalopathy and early death associated with an Ashkenazi-Jewish founder mutation in the Hikeshi gene.; Authors - Simon Edvardson
human	WB	Int J Mol Med; HIKESHI silencing can enhance mild hyperthermia sensitivity in human oral squamous cell carcinoma HSC‑3 cells.; Authors - Yoshiaki Tabuchi ; KD Validated
human,mouse	WB,IHC	Pharmaceutics; Role of HIKESHI on Hyperthermia for Castration-Resistant Prostate Cancer and Application of a Novel Magnetic Nanoparticle with Carbon Nanohorn for Magnetic Hyperthermia; Authors - Takashi Nagai ; KD Validated
mouse	WB	Front Genet; Nuclear Accumulation of HSP70 in Mouse Skeletal Muscles in Response to Heat Stress, Aging, and Unloading With or Without Reloading.; Authors - Antonios Apostolopoulos