BrdU Monoklonaler Antikörper

BrdU Monoklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

65

CloneNo.

1B10E12

Kat-Nr. : 66241-1-Ig

Synonyme

5-bromo-2-deoxyuridine, BrDu, BrdUrd, Bromodeoxyuridine, BUdR

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• Galerie der Validierungsdaten
• Proteintech vs. Other Vendors

• 

  IHC staining of mouse spleen using 66241-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide (with/without BrdU feeding) using 66241-1-Ig (BrDu Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 66241-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (with/without BrdU feeding) slide using 66241-1-Ig (BrDu Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 66241-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (with/without BrdU feeding) slide using 66241-1-Ig (BrDu Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of L-929 using 66241-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed L-929 cells using BrdU antibody (66241-1-Ig, Clone: 1B10E12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 66241-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 66241-1-Ig (BrdU antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 66241-1-Ig

  Immunofluorescent analysis of HeLa cells (treated with 0.03 mg/ml BrdU for 2 hours) using 66241-1-Ig(BrdU antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, L-929-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:200-1:1000
• Immunfluoreszenz (IF): IF : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 12 publications below
• IF: See 40 publications below
• FC: See 1 publications below

Produktinformation

66241-1-Ig bindet in IHC, IF, FC, ELISA BrdU und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Verbindung
• Vollständiger Name: BrdU
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has 0mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.

Protocol for IHC:
https://www.ptgcn.com/protocol/66241-1-IgIHC.pdf

Protocol for IF:
https://www.ptgcn.com/protocol/66241-1-IgIF.pdf

Publikationen

Species	Application	Title
mouse	IHC,IF	Nat Cell Biol; LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.; Authors - Pingping Zhu
mouse	IF	Sci Transl Med; FoxO transcription factors modulate autophagy and proteoglycan 4 in cartilage homeostasis and osteoarthritis.; Authors - Tokio Matsuzaki
mouse	IF	Cell Res; A novel m6A reader Prrc2a controls oligodendroglial specification and myelination.; Authors - Rong Wu
human	IF	Nat Commun; MRNIP condensates promote DNA double-strand break sensing and end resection.; Authors - Yun-Long Wang
mouse	IF	Nat Commun; Replication stress triggers microsatellite destabilization and hypermutation leading to clonal expansion in vitro.; Authors - Yusuke Matsuno
mouse	IHC	Bone Res; Single cell analysis reveals inhibition of angiogenesis attenuates the progression of heterotopic ossification in Mkx-/- mice.; Authors - Junxin Lin

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