BUB1 Polyklonaler Antikörper

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• KD/KO Validated

BUB1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 13330-1-AP

Synonyme

Mitotic checkpoint serine/threonine-protein kinase BUB1, hBUB1, EC:2.7.11.1, BUB1L, BUB1A

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Galerie der Validierungsdaten

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  WB analysis of MKN-45 using 13330-1-AP

  MKN-45 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 13330-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13330-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 13330-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of IMR-90 cells using 13330-1-AP

  IF results of BUB1 anitbody with IMR-90 cells by M.S. Grasiella Angelina Andriani Cristina Montagna Lab at Albert Einstein College of Medicine-Bronx - NY.

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  IF Staining of MDCK using 13330-1-AP

  Immunofluorescent analysis of MDCK cells using 13330-1-AP (BUB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: IMR-90-Zellen, MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Produktinformation

13330-1-AP bindet in WB, IHC, IF/ICC, ELISA BUB1 und zeigt Reaktivität mit human, Hund

• Getestete Reaktivität: human, Hund
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BUB1 fusion protein Ag4138
• Vollständiger Name: budding uninhibited by benzimidazoles 1 homolog (yeast)
• Berechnetes Molekulargewicht: 1085 aa, 122 kDa
• Beobachtetes Molekulargewicht: 45 kDa, 120 kDa
• GenBank-Zugangsnummer: BC028201
• Gene symbol: BUB1
• Gene ID (NCBI): 699
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Bub1 is an evolutionarily conserved mitotic checkpoint control protein that is present in diverse organisms including yeast and humans. Bub1 is a serine/threonine protein kinase and is required for recruitment of Mad1, Mad2, Bub3, and CENP-E to kinetochores. Full-length Bub1 protein was cleaved to an 45 kDa fragment and an intermediate fragment of 90 kDa, only detected at early stages of apoptosis execution, indicating cleavage of Bub1 in the amino- and carboxy-terminal regions of the protein(PMID:15818396).

Publikationen

Species	Application	Title
human		Nucleic Acids Res; ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.; Authors - Fengjie Yuan
human	IHC,IF	Cancer Sci; Stathmin guides personalized therapy in oral squamous cell carcinoma.; Authors - Wu-Tong Ju
Carassius auratus	WB	Front Bioeng Biotechnol; A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125 via Enhancement of Endomitosis in Fish.; Authors - Wenting Xu
human	WB	J Exp Clin Cancer Res; Circ_0004087 interaction with SND1 promotes docetaxel resistance in prostate cancer by boosting the mitosis error correction mechanism.; Authors - Liang Chen
human	WB	Front Pharmacol; Identification and verification of hub genes associated with the progression of non-small cell lung cancer by integrated analysis; Authors - Xie Mengyan
human	WB,IHC	Thorac Cancer; Budding uninhibited by benzimidazoles 1 overexpression is associated with poor prognosis and malignant phenotype: A promising therapeutic target for lung adenocarcinoma; Authors - Rui Chen ; KD Validated