BSEP Monoklonaler Antikörper

BSEP Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

3C11D5

Kat-Nr. : 67512-1-Ig

Synonyme

ABC16, ABCB11, Bile salt export pump, BRIC2, BSEP, PFIC 2, PFIC2, PGY4, SPGP

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Galerie der Validierungsdaten

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  WB analysis using 67512-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67512-1-Ig (BSEP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 67512-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67512-1-Ig (BSEP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 67512-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67512-1-Ig (BSEP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig lung using 67512-1-Ig

  pig lung tissue were subjected to SDS PAGE followed by western blot with 67512-1-Ig (BSEP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67512-1-Ig

  Unboiled HepG2 cell lysates, unboiled A549 cell lysates, unboilled HeLa cell lysates were subjected to SDS PAGE followed by western blot with 67512-1-Ig (BSEP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 67512-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67512-1-Ig (BSEP antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 67512-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67512-1-Ig (BSEP antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 67512-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67512-1-Ig (BSEP antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 67512-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67512-1-Ig (BSEP antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 67512-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BSEP antibody (67512-1-Ig, Clone: 3C11D5 ) at dilution of 1:10000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, A549-Zellen, Hausschwein-Lungengewebe, HeLa-Zellen, Mauslebergewebe, Rattenlebergewebe, ungekochte HepG2-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:8000-1:32000
• Immunfluoreszenz (IF): IF : 1:5000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

67512-1-Ig bindet in WB, IHC, IF, ELISA BSEP und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BSEP fusion protein Ag29135
• Vollständiger Name: ATP-binding cassette, sub-family B (MDR/TAP), member 11
• Berechnetes Molekulargewicht: 146 kDa
• Beobachtetes Molekulargewicht: 150-160 kDa
• GenBank-Zugangsnummer: NM_003742
• Gene symbol: ABCB11
• Gene ID (NCBI): 8647
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ABCB11, also named as BSEP, belongs to the ABC transporter superfamily. ABCB11 involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. Mutation of ABCB11 will cause the progressive familial intrahepatic cholestasis type 2 (PFIC2) and the benign recurrent intrahepatic cholestasis type 2 (BRIC2). Genetic variations in ABCB11 may play a role in drug-induced cholestasis causing liver damage. The calculated MW of ABCB11 is 146 kDa, 67512-1-Ig can detect bands around 150 kDa. It's reported that ABCB11 has several N-glycosylation sites, the observed higher molecular mass may due to posttranslational N-glycosylation. (PMID: 9545351, 24359682)

Publikationen

Species	Application	Title
mouse	IHC	Front Pharmacol; Vitamin C and vitamin D3 alleviate metabolic-associated fatty liver disease by regulating the gut microbiota and bile acid metabolism via the gut-liver axis; Authors - Qingling Chen
mouse	WB	Appl Biochem Biotechnol; Sphincter of Oddi Dysfunction Induces Gallstone by Inhibiting the Expression of ABCB11 via PKC-α; Authors - Lichao Zhu