BRSK2 Polyklonaler Antikörper

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• KD/KO Validated

BRSK2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11589-1-AP

Synonyme

BR serine/threonine kinase 2, BRSK2, C11orf7, PEN11B, SAD1, STK29

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Galerie der Validierungsdaten

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  WB analysis using 11589-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11589-1-AP (BRSK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11589-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11589-1-AP (BRSK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11589-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11589-1-AP (BRSK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11589-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11589-1-AP (BRSK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maushodengewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

11589-1-AP bindet in WB, IP, IF, ELISA BRSK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BRSK2 fusion protein Ag2195
• Vollständiger Name: BR serine/threonine kinase 2
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 80-88 kDa
• GenBank-Zugangsnummer: BC024291
• Gene symbol: BRSK2
• Gene ID (NCBI): 9024
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BRSK2 is highly conserved from invertebrates to human, plays a key role in polarization of neurons and has been identified with different names: SAD1 is its closest homolog in C. elegans, serine/threonine kinase SADA alfa in rat, brain-selective kinase 2 and SAD-B in mouse and hsSAD1 and SAD1B in humans(PMID:16165222) . In rat and mouse, BRSK2 is only detected in the brain and at low levels in the testis .It has some isoforms produced by alternative splicing with the molecular mass of 68-84 kDa. This protein has a conserved function throughout the evolution.

Publikationen

Species	Application	Title
rat	WB	J Biol Chem; Specification of neuronal polarity regulated by local translation of CRMP2 and Tau via the mTOR-p70S6K pathway.; Authors - Morita Tsuyoshi T
human,rat	WB	J Neurochem; Phosphorylation of microtubule-associated protein tau by AMPK-related kinases.; Authors - Yoshida Hirotaka H
rat	WB,IP	Endocrinology; SAD-A Promotes Glucose-Stimulated Insulin Secretion Through Phosphorylation and Inhibition of GDIα in Male Islet β Cells.; Authors - Jia Nie ; KD Validated
human,mouse	WB,IF,IP	Cancer Lett; SOD1-high fibroblasts derived exosomal miR-3960 promotes cisplatin resistance in triple-negative breast cancer by suppressing BRSK2-mediated phosphorylation of PIMREG; Authors - Kangdi Li ; KD Validated