BRSK1 Polyklonaler Antikörper

BRSK1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 12673-1-AP

Synonyme

BR serine/threonine kinase 1, BRSK1, KIAA1811, SAD1, SAD1 kinase

---

Galerie der Validierungsdaten

• 

  WB analysis using 12673-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12673-1-AP (BRSK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12673-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12673-1-AP (BRSK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12673-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12673-1-AP (BRSK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12673-1-AP

  IP result of anti-BRSK1 (IP:12673-1-AP, 4ug; Detection:12673-1-AP 1:500) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of human ovary tumor using 12673-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12673-1-AP (BRSK1 antibody) at dilution of 1:50 (under 10x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

12673-1-AP bindet in WB, IP, IHC, ELISA BRSK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BRSK1 fusion protein Ag3357
• Vollständiger Name: BR serine/threonine kinase 1
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 85-90 kDa
• GenBank-Zugangsnummer: BC016681
• Gene symbol: BRSK1
• Gene ID (NCBI): 84446
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BRSK1(Brain-specific serine/threonine-protein kinase 1) is also named as KIAA1811, SAD1, SADB and belongs to the SNF1 subfamily. BRSKs are crucial for establishing neuronal polarity, and BRSK1 has also been shown to regulate neurotransmitter release presynaptically. BRSK1 is a serine-threonine kinases specifically expressed in the brain, and activated by LKB1-mediated phosphorylation of a threonine residue at their T-loop. (PMID:22020259). It has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
rat	WB	J Biol Chem; Specification of neuronal polarity regulated by local translation of CRMP2 and Tau via the mTOR-p70S6K pathway.; Authors - Morita Tsuyoshi T
goat	WB	Cell Death Discov; Molecular mechanism underlying miR-204-5p regulation of adipose-derived stem cells differentiation into cells from three germ layers; Authors - Zhimin Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Ester (Verified Customer) (04-21-2021)

Worked well for Immunofluoresence.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: OVCAR8,PEO4