BRD3 Polyklonaler Antikörper

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• KD/KO Validated

BRD3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

28

Rezensionen (1)

Kat-Nr. : 11859-1-AP

Synonyme

RING3-like protein, RING3L, RING3 like protein, ORFX, KIAA0043

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Galerie der Validierungsdaten

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  WB analysis using 11859-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11859-1-AP (BRD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11859-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11859-1-AP (BRD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 11859-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11859-1-AP (BRD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11859-1-AP

  IP result of anti-BRD3 (IP:11859-1-AP, 4ug; Detection:11859-1-AP 1:500) with HeLa cells lysate 4000ug.

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  IHC staining of human prostate cancer using 11859-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11859-1-AP (BRD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 11859-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11859-1-AP (BRD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11859-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11859-1-AP (BRD3 antibody) at dilution of 1:25 (under 10x lens).

• 

  IF Staining of HeLa using 11859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11859-1-AP (BRD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, COLO 320-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 26 publications below
• IHC: See 2 publications below

Produktinformation

11859-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA BRD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BRD3 fusion protein Ag2433
• Vollständiger Name: bromodomain containing 3
• Berechnetes Molekulargewicht: 556 aa, 61 kDa
• Beobachtetes Molekulargewicht: 95-105 kDa
• GenBank-Zugangsnummer: BC032124
• Gene symbol: BRD3
• Gene ID (NCBI): 8019
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BRD3 was belongs to the BET family that a small group of unique, highly conserved bromodomain-containing genes with four members in mouse and human. There are BRD2, BRD3, BRD4 and BRDT. And BET family proteins all localize in the nucleus, and contain two tandems N-terminal BRDs, an extra-terminal (ET) domain and a more divergent C-terminal recruitment domain (PMID:25849938). BRD2, BRD3, and BRD4 play key roles in transcription of genes required for both inflammation and cancer. Northern hybridization revealed that BRD3 is most abundant in testis, ovary, placenta, uterus, and brain(PMID:15261828; PMID:27451123). The antibody could recognize the common about 100 kDa protein in applications as the papers (PMID:29437854; 22595521).

Publikationen

Species	Application	Title
human	WB	Nat Med; Prostate cancer-associated SPOP mutations confer resistance to BET inhibitors through stabilization of BRD4.; Authors - Xiangpeng Dai
human,mouse		Mol Cell; A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis; Authors - Kaiwen Bao
human	WB	Sci Adv; Light-induced control of protein destruction by opto-PROTAC.; Authors - Jing Liu
human	WB	J Am Chem Soc; Cancer Selective Target Degradation by Folate-Caged PROTACs.; Authors - Jing Liu
human	WB	Nat Commun; Stromal induction of BRD4 phosphorylation Results in Chromatin Remodeling and BET inhibitor Resistance in Colorectal Cancer.; Authors - Wenyu Wang
human	WB	Nat Commun; A proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues.; Authors - Satoshi Yamanaka

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zehua (Verified Customer) (02-06-2019)

Works well on fractionation lysates of MCF-7 cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF-7

BRD3 Antibody Western Blot, validation (1:1000 dilution) in MCF-7 (Cat no:11859-1-AP)