BRAF Polyklonaler Antikörper

BRAF Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 20899-1-AP

Synonyme

B RAF1, BRAF, BRAF1, p94, Proto oncogene B Raf, RAFB1

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Galerie der Validierungsdaten

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  WB analysis using 20899-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20899-1-AP (BRAF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20899-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20899-1-AP (BRAF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human malignant melanoma using 20899-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 20899-1-AP (BRAF Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human malignant melanoma using 20899-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 20899-1-AP (BRAF Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 20899-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20899-1-AP (BRAF Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 20899-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20899-1-AP (BRAF Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human thyroid cancer using 20899-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 20899-1-AP (BRAF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 20899-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 20899-1-AP (BRAF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 20899-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 20899-1-AP (BRAF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 20899-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BRAF antibody (20899-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes malignes Melanomgewebe, humanes Hodengewebe, humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: NIH/3T3-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below
• IHC: See 1 publications below

Produktinformation

20899-1-AP bindet in WB, IHC, IF, ELISA BRAF und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BRAF fusion protein Ag15014
• Vollständiger Name: v-raf murine sarcoma viral oncogene homolog B1
• Berechnetes Molekulargewicht: 766 aa, 84 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC101757
• Gene symbol: BRAF
• Gene ID (NCBI): 673
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

B-Raf proto-oncogene serine/threonine kinase(BRAF), is a protein belonging to the raf/mil family of serine/threonine protein kinases. BRAF plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. BRAF is associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. BRAF is also associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. BRAF plays a role in the PD-1/PDL-1 pathway. In some cancers, BRAF is activated by rearrangements that fuse its kinase domain to 5' partner genes and then has different molecular weights (PMID: 23890088). BRAF has several isoforms, the calculated molecular weight of BRAF is 84 kDa, but the observed molecular weight is about 65 kDa(isoform).

Publikationen

Species	Application	Title
mouse	WB	Acta Pharmacol Sin; 1-Indanone retards cyst development in ADPKD mouse model by stabilizing tubulin and down-regulating anterograde transport of cilia; Authors - Xiao-Wei Li
human	WB	J Eur Acad Dermatol Venereol; Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi.; Authors - T Yuan
human	WB	Biochim Biophys Acta Mol Cell Res; IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5; Authors - Junfeng Du
human	WB	Front Immunol; Immune Dysfunction Mediated by the ceRNA Regulatory Network in Human Placenta Tissue of Intrahepatic Cholestasis Pregnancy.; Authors - Yuya Wang
human	WB	Life Sci; Network pharmacology and molecular docking reveal the mechanism of Scopoletin against non-small cell lung cancer.; Authors - Chong Yuan
chicken	WB	Gene; WT1 suppresses follicle-stimulating hormone-induced progesterone secretion by regulating ERK1/2 pathway in chicken preovulatory granulosa cells.; Authors - Shuixin Tang