BORIS Polyklonaler Antikörper

BORIS Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11074-2-AP

Synonyme

CTCFL, CTCF-like protein, CTCF T, CTCF paralog, CT27

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Galerie der Validierungsdaten

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  WB analysis of PC-3 using 11074-2-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11074-2-AP (BORIS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 11074-2-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 11074-2-AP (BORIS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11074-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11074-2-AP (BORIS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 11074-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11074-2-AP (BORIS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 11074-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11074-2-AP (BORIS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 11074-2-AP

  Immunofluorescent analysis of SH-SY5Y7 cells, using CTCFL antibody 11074-2-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HeLa using 11074-2-AP

  Immunofluorescent analysis of HeLa cells using 11074-2-AP (BORIS antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11074-2-AP bindet in WB, IHC, IF/ICC, ELISA BORIS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BORIS fusion protein Ag1547
• Vollständiger Name: CCCTC-binding factor (zinc finger protein)-like
• Berechnetes Molekulargewicht: 82 kDa, 75 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC130486
• Gene symbol: BORIS/CTCFL
• Gene ID (NCBI): 140690
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTCFL, also named BORIS (for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. Present polyclonal anti-CFCFL antibody(11074-2-AP) is produced by immunizing animals with part of C-terminal chain of CTCFL and detect a 75-kDa band and a 55-kDa band.