BMP6 Polyklonaler Antikörper

BMP6 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55421-1-AP

Synonyme

BMP 6, BMP6, BMP-6, bone morphogenetic protein 6, VG 1 R, VG 1 related protein, VGR, VGR 1, VGR1

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Galerie der Validierungsdaten

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  WB analysis using 55421-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55421-1-AP (BMP6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 55421-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55421-1-AP (BMP6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 55421-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55421-1-AP (BMP6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 55421-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55421-1-AP (BMP6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 55421-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55421-1-AP (BMP6 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 55421-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55421-1-AP (BMP6 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HL-60-Zellen, Mauslebergewebe, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

55421-1-AP bindet in WB, ELISA BMP6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: bone morphogenetic protein 6
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: NM_001718
• Gene symbol: BMP6
• Gene ID (NCBI): 654
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Bone morphogenetic protein 6 (BMP6) is also named as VG-1-related protein (VG-1-R, VGR-1) and VGR, and belongs to the TGF-beta family. (PMID:33878365). Growth factor of the TGF-beta superfamily plays essential roles in many developmental processes including cartilage and bone formation (PMID:31019025). BMP6 participates in iron homeostasis, inhibits invasion by increasing adhesions and cell-cell type interactions and induces angiogenesis directly on vascular endothelial cells. BMP6 is coded by a tumor suppressor gene whose subexpression is related to the development and cancer progression; during neoplastic processes, methylation is the main mechanism by which gene silencing occurs (PMID:34706618). BMP6 plays an important role in the regulation of hepcidin/HAMP expression and iron metabolism by acting as a ligand for hemojuvelin/HJV (PMID:26582087). BMP6 initiates the canonical BMP signaling cascade by associating with type I receptor ACVR1 and type II receptor ACVR2B (PMID:18070108).