BMP2 Monoklonaler Antikörper

BMP2 Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human und mehr (2)

Anwendung

WB, IHC, IF, Cell treatment, ELISA

Konjugation

Unkonjugiert

Publikationen

35

CloneNo.

1C5C2

Kat-Nr. : 66383-1-Ig

Synonyme

BMP 2, BMP 2A, BMP2, BMP2A, bone morphogenetic protein 2, Bone morphogenetic protein 2A

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Galerie der Validierungsdaten

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  WB analysis of pig bone marrow using 66383-1-Ig

  pig bone marrow tissue were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66383-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 66383-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 66383-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66383-1-Ig (BMP2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 66383-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66383-1-Ig (BMP2 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Knochenmarkgewebe, Caco-2-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 29 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

66383-1-Ig bindet in WB, IHC, IF, Cell treatment, ELISA BMP2 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BMP2 fusion protein Ag13501
• Vollständiger Name: bone morphogenetic protein 2
• Berechnetes Molekulargewicht: 396 aa, 44 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC069214
• Gene symbol: BMP2
• Gene ID (NCBI): 650
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP2 is synthesized as a 60 kDa precursor that is processed in the secretory pathway to a small 18 kDa monomer; 2 monomers then associate to form the active 30 kDa homodimer, which binds to its receptor. There is also a 40-45 kDa form of BMP2, as an amino-terminal propeptide. BMP2 can induce bone formation and regeneration during early embryonic development. It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction.

Publikationen

Species	Application	Title
human	WB	ACS Appl Mater Interfaces; Nitrogen-Doped Multiwalled Carbon Nanotubes Enhance Bone Remodeling through Immunomodulatory Functions.; Authors - Haifang Li
human	WB	Angiogenesis; Linalool inhibits the angiogenic activity of endothelial cells by downregulating intracellular ATP levels and activating TRPM8.; Authors - Vivien Becker
mouse	WB	Phytother Res; Bavachinin selectively modulates PPAR γ and maintains bone homeostasis in Type 2 Diabetes; Authors - Jingwen Liu
human	WB	Oncogenesis; Neuron-specific enolase promotes stem cell-like characteristics of small-cell lung cancer by downregulating NBL1 and activating the BMP2/Smad/ID1 pathway.; Authors - Lin Lu
rat	WB	J Hypertens; Saturated fatty acid promotes calcification via suppressing SIRT6 expression in vascular smooth muscle cells; Authors - Yafen Tao
human	WB	Front Oncol; Increased Methyl-CpG-Binding Domain Protein 2 Promotes Cigarette Smoke-Induced Pulmonary Hypertension.; Authors - Jixing Wu