BLVRA Polyklonaler Antikörper

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• KD/KO Validated

BLVRA Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10775-1-AP

Synonyme

BVR A, BVR, BLVR, Biliverdin-IX alpha-reductase, biliverdin reductase A

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Galerie der Validierungsdaten

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  WB analysis using 10775-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10775-1-AP (BLVRA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10775-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10775-1-AP (BLVRA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 10775-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10775-1-AP (BLVRA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10775-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10775-1-AP (BLVRA antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas using 10775-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10775-1-AP (BLVRA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 10775-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10775-1-AP (BLVRA antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 10775-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10775-1-AP (BLVRA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 10775-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10775-1-AP (BLVRA antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of L02 using 10775-1-AP

  Immunofluorescent analysis of L02 cells using 10775-1-AP (BLVRA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, HuH-7-Zellen, L02-Zellen, Mauslebergewebe, PC-3-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

10775-1-AP bindet in WB, IHC, IF/ICC, ELISA BLVRA und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BLVRA fusion protein Ag1166
• Vollständiger Name: biliverdin reductase A
• Berechnetes Molekulargewicht: 33.2 kDa
• Beobachtetes Molekulargewicht: 37-40 kDa, 66 kDa
• GenBank-Zugangsnummer: BC008456
• Gene symbol: BLVRA
• Gene ID (NCBI): 644
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BLVRA(Biliverdin reductase A) is also named as BLVR, BVR and belongs to the biliverdin reductase subfamily.It catalyze the conversion of biliverdin to bilirubin in the presence of NADPH or NADH.This protein can exsit as a dimer(PMID:11773068). Defects in BLVRA are the cause of hyperbiliverdinemia (HBLVD).

Publikationen

Species	Application	Title
human	WB,IHC	Cancer Manag Res; Biliverdin Reductase A (BLVRA) Promotes Colorectal Cancer Cell Progression by Activating the Wnt/β-Catenin Signaling Pathway.; Authors - Haiyan Mao ; KD Validated
human	IF	Arch Biochem Biophys; Biliverdin reductase deficiency triggers an endothelial-to-mesenchymal transition in human endothelial cells.; Authors - Damian Klóska
human	WB	EXCLI J; Induction of cell apoptosis by biliverdin reductase inhibitor in MCF-7 and MDA-MB-468 breast cancer cell lines: Experimental and in silico studies.; Authors - Seyedeh Zahra Shahrokhi