BHLHE41 Polyklonaler Antikörper

BHLHE41 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 12688-1-AP

Synonyme

hDEC2, Enhancer-of-split and hairy-related protein 1, DEC2, Class E basic helix-loop-helix protein 41, Class B basic helix-loop-helix protein 3

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Galerie der Validierungsdaten

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  WB analysis using 12688-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12688-1-AP (BHLHE41 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 12688-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using BHLHE41 antibody (12688-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

12688-1-AP bindet in WB, IHC, IF/ICC, IP, chIP, ELISA BHLHE41 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BHLHE41 fusion protein Ag3381
• Vollständiger Name: basic helix-loop-helix family, member e41
• Berechnetes Molekulargewicht: 482 aa, 51 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC025968
• Gene symbol: BHLHE41
• Gene ID (NCBI): 79365
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BHLHE41 (basic helix-loop-helix family member e41), also called SHARP1 and DEC2, is a member of the BHLH family proteins that are involved in differentiation, circadian rhythms and the response to hypoxia. In esophageal, osteosarcomas, prostate, breast, and endometrial cancer cells, BHLHE41 functions as an anti-apoptotic factor and promotes cell proliferation and invasiveness. It suppresses cell proliferation and metastasis, and also facilitates pancreatic as well as gastric cancer cell apoptosis. BHLHE41 has an important role in the proliferation of glioblastoma and couldserve as a novel candidate for targeted therapy of glioblastoma (PMID: 31616182).

Publikationen

Species	Application	Title
human	chIP	Nat Commun; The basic helix-loop-helix transcription factor SHARP1 is an oncogenic driver in MLL-AF6 acute myelogenous leukemia.; Authors - Akihiko Numata
rat	WB	FASEB J; Persistent myopathy despite release of partial obstruction: in vivo reversal of dysfunction and transcriptional responses using rapamycin.; Authors - Annette Schröder
human	WB	Biochem Biophys Res Commun; Loss of circadian protein TIMELESS accelerates the progression of cellular senescence.; Authors - Xiaomeng Shen
human	WB, IHC	J Exp Clin Cancer Res; DEC2 expression is positively correlated with HIF-1 activation and the invasiveness of human osteosarcomas.; Authors - Tu Hu
human	WB,IHC	J Exp Clin Cancer Res; Inhibition of DEC2 is necessary for exiting cell dormancy in salivary adenoid cystic carcinoma.; Authors - Xiao Yang
human	WB,IF	Cell Death Dis; Nanog mediated by FAO/ACLY signaling induces cellular dormancy in colorectal cancer cells.; Authors - Meng Zhang