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Bestrophin-1 Monoklonaler Antikörper

Bestrophin-1 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

4D5F1

Kat-Nr. : 60326-1-Ig

Synonyme

ARB, BEST, BEST1, bestrophin 1, Bestrophin-1, BMD, TU15B, VMD2

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of Y79 cells using Bestrophin-1 Monoclonal antibody (60326-1-Ig)

    WB analysis of Y79 using 60326-1-Ig

    Y79 cells were subjected to SDS PAGE followed by western blot with 60326-1-Ig (Bestrophin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using Bestrophin-1 Monoclonal antibody (60326-1-Ig)

    WB analysis of Transfected HEK-293 using 60326-1-Ig

    WB result of Bestrophin-1 antibody (60326-1-Ig; 1:1000; room temperature for 1.5 hours) with negatove control and Bestrophin-1 overexpressed Transfected HEK-293 cells.

  • Immunofluorescence (IF) / fluorescent staining of Y79 cells using Bestrophin-1 Monoclonal antibody (60326-1-Ig)

    IF Staining of Y79 using 60326-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Y79 cells using Bestrophin-1 antibody (60326-1-Ig, Clone: 4D5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBY79-Zellen, Transfizierte HEK-293-Zellen
Erfolgreiche Detektion in IFY79-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunfluoreszenz (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IFSee 1 publications below

Produktinformation

60326-1-Ig bindet in WB, IF, ELISA Bestrophin-1 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Bestrophin-1 fusion protein Ag15129
Vollständiger Name bestrophin 1
Berechnetes Molekulargewicht 585 aa, 68 kDa
Beobachtetes Molekulargewicht 68 kDa
GenBank-ZugangsnummerBC015220
Gene symbol BEST1
Gene ID (NCBI) 7439
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Bestrophin-1 (BEST1) is a 68 kDa transmembrane protein which belongs to the bestrophin family of anion channels. It is predominantly expressed in the basolateral membrane of the retinal pigment epithelium. Studies show that bestrophin 1 functions as a Ca(2+)-dependent Cl(-) channel and modulator of voltage-dependent L-type Ca(2+) channels. It may also contribute to the basolateral cell conductance in airway epithelial cells. This protein is encoded by the VMD2 gene. Mutations in VMD2 can lead to different types of retinal or macular degenerations, including Best vitelliform macular dystrophy (BMD), autosomal recessive bestrophinopathy (ARB), autosomal dominant vitreoretinochoroidopathy (ADVIRC) and adult-onset vitelliform macular dystrophy (AVMD).

Protokolle

Produktspezifische Protokolle
WB protocol for Bestrophin-1 antibody 60326-1-IgProtokoll herunterladen
IF protocol for Bestrophin-1 antibody 60326-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIF

Cell Death Dis

Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration

Authors - Xiaoman Zhu
View Article