BCOR Polyklonaler Antikörper

BCOR Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Rezensionen (1)

Kat-Nr. : 12107-1-AP

Synonyme

ANOP2, BCOR, KIAA1575, MAA2, MCOPS2

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Galerie der Validierungsdaten

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  WB analysis using 12107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12107-1-AP (BCOR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12107-1-AP (BCOR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 12107-1-AP

  IP result of anti-BCOR (IP:12107-1-AP, 4ug; Detection:12107-1-AP 1:5000) with HEK-293 cells lysate 920 ug.

• 

  IHC staining of human breast cancer using 12107-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12107-1-AP (BCOR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12107-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12107-1-AP (BCOR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, K-562-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Produktinformation

12107-1-AP bindet in WB, IP, IHC, CoIP, ChIP, ELISA BCOR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BCOR fusion protein Ag2752
• Vollständiger Name: BCL6 co-repressor
• Berechnetes Molekulargewicht: 1703 aa, 186 kDa
• Beobachtetes Molekulargewicht: 186 kDa
• GenBank-Zugangsnummer: BC009675
• Gene symbol: BCOR
• Gene ID (NCBI): 54880
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCOR, a corepressor of BCL6, binds to transcriptional factors and specifically inhibits gene expression. BCOR plays a key role in the early embryonic development, mesenchymal stem cell function and hematopiesis. BCOR can associate with HDACs, the polycomb group protein, polycomb group Ring finger protein1/nervous system polycomb1 (PCGF1/NSPC1), histone demethylase, F-box- and leucine-rich repeat protein10(FBXL10). Via repression of TFAP2A acts as a negative regulator of osteo-dentiogenic capacity in adult stem

Publikationen

Species	Application	Title
	WB, IHC	Nat Genet; Consistent in-frame internal tandem duplications of BCOR characterize clear cell sarcoma of the kidney.; Authors - Hitomi Ueno-Yokohata
human	WB	Nucleic Acids Res; Bimodal regulation of the PRC2 complex by USP7 underlies tumorigenesis.; Authors - Dongxue Su
mouse	WB	Int J Mol Sci; BCOR Internal Tandem Duplication Expression in Neural Stem Cells Promotes Growth, Invasion, and Expression of PRC2 Targets.; Authors - Satoshi Nakata
mouse	WB	Sci Rep; Loss of Polycomb Group Protein Pcgf1 Severely Compromises Proper Differentiation of Embryonic Stem Cells.; Authors - Yun Yan
		Cell Prolif; Secreted frizzled-related protein 2 promotes the osteo/odontogenic differentiation and paracrine potentials of stem cells from apical papilla under inflammation and hypoxia conditions.; Authors - Haoqing Yang
human	IHC	J Clin Pathol; Novel exon-exon breakpoint in CIC-DUX4 fusion sarcoma identified by anchored multiplex PCR (Archer FusionPlex Sarcoma Panel).; Authors - Benjamin Nathanael Loke

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sid (Verified Customer) (05-13-2019)

Works well for mouse hematopoetic cells, some strong non specific bands ~ 150 kD and ~100 kD, however can be reasonably resolved on 4-15% gel.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Mouse hematopoetic