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BCLAF1 Monoklonaler Antikörper

BCLAF1 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1D6G7

Kat-Nr. : CL594-67860

Synonyme

BCLAF1, bK211L9.1, BTF, KIAA0164

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated BCLAF1 Monoclonal antibody (CL594-67860)

    FC experiment of HepG2 using CL594-67860

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human BCLAF1 (CL594-67860, Clone:1D6G7) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Geprüfte Anwendungen

Erfolgreiche Detektion in FCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67860 bindet in FC (Intra) BCLAF1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen BCLAF1 fusion protein Ag25210
Vollständiger Name BCL2-associated transcription factor 1
Berechnetes Molekulargewicht 920 aa, 106 kDa
Beobachtetes Molekulargewicht145 kDa
GenBank-ZugangsnummerBC132780
Gene symbol BCLAF1
Gene ID (NCBI) 9774
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCLF1, also named as Bcl-2-associated transcription factor 1, is a 920 amino acid protein, which Interacts with Bcl-2 related proteins, EMD, with the adenovirus E1B 19 kDa protein and with DNA. BCLF1 as a death-promoting transcriptional repressor may be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. The calculated molecular weight of BCLF1 is a 110 kDa, but the modified BCLF1 protein is about 140-150 kDa.

Protokolle

Produktspezifische Protokolle
FC protocol for CL594 BCLAF1 antibody CL594-67860Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen