BCKDHB Polyklonaler Antikörper

BCKDHB Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 13685-1-AP

Synonyme

BCKDHB, E1B

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Galerie der Validierungsdaten

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  WB analysis using 13685-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13685-1-AP (BCKDHB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 13685-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 13685-1-AP (BCKDHB Antibody) at dilution of 1:350 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 13685-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13685-1-AP (BCKDHB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13685-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13685-1-AP (BCKDHB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 13685-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13685-1-AP (BCKDHB antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 13685-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13685-1-AP (BCKDHB antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Rattenlebergewebe, Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• RIP: See 1 publications below

Produktinformation

13685-1-AP bindet in WB, RIP, IHC, ELISA BCKDHB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BCKDHB fusion protein Ag4552
• Vollständiger Name: branched chain keto acid dehydrogenase E1, beta polypeptide
• Berechnetes Molekulargewicht: 392 aa, 43 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC040139
• Gene symbol: BCKDHB
• Gene ID (NCBI): 594
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Branched-chain alpha-keto acid dehydrogenase E1 component beta chain (BCKDHB; BCKDE1B; BCKDH E1-beta) is a subunit of the BCKDH complex, which is a mitochondrial enzyme in the degradation pathway for branched-chain amino acids (BCAA). Together with BCKDHA, BCKDHB forms the E1 subunit of this complex, whereas DBT and DLD are the E2 and E3 subunits, respectively. A deficiency of the BCKDH complex in humans causes maple syrup urine disease (MSUD), a severe neurometabolic disorder diagnosed by the detection alloisoleucine in plasma (MIM 248600). (PMID: 30709776)

Publikationen

Species	Application	Title
sheep	WB	Anim Sci J; MiR-330-5p negatively regulates ovine preadipocyte differentiation by targeting branched-chain aminotransferase 2.; Authors - Tao Shi
mouse	WB	Cell Rep; SIRT4 is an early regulator of branched-chain amino acid catabolism that promotes adipogenesis.; Authors - Elma Zaganjor
human	WB	Cell Rep; Branched-chain amino acid catabolism breaks glutamine addiction to sustain hepatocellular carcinoma progression; Authors - Dongdong Yang
human	IHC	Nat Commun; AMPK induces degradation of the transcriptional repressor PROX1 impairing branched amino acid metabolism and tumourigenesis; Authors - Yanan Wang
mouse	WB	J Thromb Haemost; Branched-chain amino acids promote thrombocytopoiesis by activating mTOR signaling; Authors - Haojie Jiang
mouse	WB,RIP	Nucleic Acids Res; Mitolnc controls cardiac BCAA metabolism and heart hypertrophy by allosteric activation of BCKDH; Authors - Maria Weiss