BCAT1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

BCAT1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

23

Rezensionen (2)

Kat-Nr. : 13640-1-AP

Synonyme

PNAS 121, MECA39, ECA39, EC:2.6.1.42, Branched-chain-amino-acid aminotransferase, cytosolic

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Galerie der Validierungsdaten

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  WB analysis using 13640-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13640-1-AP (BCAT1/ECA39 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13640-1-AP

  WB result of BCAT1/ECA39 antibody (13640-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCAT1/ECA39 transfected HeLa cells.

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  IHC staining of mouse brain using 13640-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13640-1-AP (BCAT1/ECA39 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13640-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13640-1-AP (BCAT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13640-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 13640-1-AP (BCAT1/ECA39 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, Neuro-2a-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 20 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Produktinformation

13640-1-AP bindet in WB, IHC, IF/ICC, ELISA BCAT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BCAT1 fusion protein Ag4574
• Vollständiger Name: branched chain aminotransferase 1, cytosolic
• Berechnetes Molekulargewicht: 320 aa, 36 kDa
• Beobachtetes Molekulargewicht: 43-45 kDa
• GenBank-Zugangsnummer: BC033864
• Gene symbol: BCAT1
• Gene ID (NCBI): 586
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCAT1, also named branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine, and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149). The 13640-1-AP antibody detects the bands around 43-45 kDa for different isoforms and higher molecular weight for phosphorylation modification of this protein.

Publikationen

Species	Application	Title
human	WB	Nat Metab; Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression; Authors - Lin Qian ; KO Validated
mouse	IHC	Nat Cell Biol; BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma.; Authors - Jin-Tao Li
human	WB, IHC	Basic Res Cardiol; Oxoeicosanoid receptor inhibition alleviates acute myocardial infarction through activation of BCAT1.; Authors - Qiong Lai
human	WB	Cancer Res; Targeting BCAT1 combined with α-ketoglutarate triggers metabolic synthetic lethality in glioblastoma.; Authors - Bo Zhang
human	WB	Cell Mol Life Sci; Regulation of branched-chain amino acid metabolism by hypoxia-inducible factor in glioblastoma.; Authors - Bo Zhang ; KO Validated
human	WB,IHC	Oncotarget; BCAT1 expression associates with ovarian cancer progression: possible implications in altered disease metabolism.; Authors - Zhi-Qiang Wang ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kis (Verified Customer) (12-08-2022)

This antibody was used for (1:1000) WB and (1:500) for IHC. We got satisfactory results with this ab.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Liver cells

BCAT1 Antibody Western Blot,Immunohistochemistry validation (1:1000 dilution) in Human Liver cells (Cat no:13640-1-AP)

FH

Iru (Verified Customer) (06-18-2020)

It works fine with FTE cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fallopian tube epithelial cells