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Brevican Polyklonaler Antikörper

Brevican Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19017-1-AP

Synonyme

BCAN, BEHAB, Brevican, Brevican core protein, CSPG7

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of human brain tissue using Brevican Polyclonal antibody (19017-1-AP)

    WB analysis of human brain using 19017-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using Brevican Polyclonal antibody (19017-1-AP)

    WB analysis of SH-SY5Y using 19017-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using Brevican Polyclonal antibody (19017-1-AP)

    WB analysis of COLO 320 using 19017-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using Brevican Polyclonal antibody (19017-1-AP)

    WB analysis of Y79 using 19017-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using Brevican Polyclonal antibody (19017-1-AP)

    IHC staining of human cerebellum using 19017-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19017-1-AP (Brevican antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using Brevican Polyclonal antibody (19017-1-AP)

    IHC staining of human cerebellum using 19017-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19017-1-AP (Brevican antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Brevican Polyclonal antibody (19017-1-AP)

    IHC staining of mouse brain using 19017-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19017-1-AP (Brevican antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBhumanes Hirngewebe, COLO 320-Zellen, SH-SY5Y-Zellen, Y79-Zellen
Erfolgreiche Detektion in IHChumanes Cerebellum-Gewebe, Maushirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

19017-1-AP bindet in WB, IHC, ELISA Brevican und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Brevican fusion protein Ag13552
Vollständiger Name brevican
Berechnetes Molekulargewicht 99 kDa
Beobachtetes Molekulargewicht 145 kDa, 72kDa, 80 kDa
GenBank-ZugangsnummerBC010571
Gene symbol BCAN
Gene ID (NCBI) 63827
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Brevican is a brain-specific proteoglycan belonging to the aggrecan family that share a number of structural features. Brevican contains a hyaluronic acid-binding domain in its N-terminus and a lectin-like domain in its C-terminus. Expression of brevican is highly specific in the brain and increases as the brain develops. It may play a role in maintaining the extracellular environment of mature brain as a major constituent of the adult brain extracellular matrix (PMID:9117262). Brevican core protein exists as a 145 kDa full-length form and a 80 kDa N terminally truncated form (PMID: 8144512). This antibody can recognize the 72 kDa isoform of target.

Protokolle

Produktspezifische Protokolle
WB protocol for Brevican antibody 19017-1-APProtokoll herunterladen
IHC protocol for Brevican antibody 19017-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen