• Überblick
  • Geprüfte Anwendungen
  • Produktinformation
  • Publikationen (18)
  • Protokolle
  • Rezensionen (1)
  • Featured Product
  • KD/KO Validated

BBS4 Polyklonaler Antikörper

BBS4 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12766-1-AP

Synonyme

Bardet-Biedl syndrome 4 protein, Bardet Biedl syndrome 4



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, Mausnierengewebe
Erfolgreiche Detektion in IHChumanes Nierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICChTERT-RPE1-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:4000
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

12766-1-AP bindet in WB, IHC, IF/ICC, ELISA BBS4 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen BBS4 fusion protein Ag3430
Vollständiger Name Bardet-Biedl syndrome 4
Berechnetes Molekulargewicht 519 aa, 58 kDa
Beobachtetes Molekulargewicht 50-60 kDa
GenBank-ZugangsnummerBC027624
Gene symbol BBS4
Gene ID (NCBI) 585
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BBS4 (Bardet-Biedl syndrome protein 4) is one of the early BBS proteins discovered, with 35 mutations reported to cause the BBS phenotype (PMID: 26762677). BBS4 undergoes localization to the centriolar satellites of centrosomes and basal body of primary cilia, where it takes part in recruiting cargo to centriolar satellites and allowing the formation of a functional centrosomal microtubule organizing center (MTOC) (PMID: 25860617). Some research has found that BBS4 expression in humans during the development of CNS and sensory organs, concerning the cardinal clinical signs of BBS (PMID: 33860840).

Protokolle

Produktspezifische Protokolle
WB protocol for BBS4 antibody 12766-1-APProtokoll herunterladen
IHC protocol for BBS4 antibody 12766-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

  • Journal Impact Factor
  • Most recent
SpeciesApplicationTitle
humanIF

Nat Commun

Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets.

Authors - Dragomir B Krastev
mouseWB,IF

PLoS Biol

Loss of Bardet-Biedl syndrome proteins causes synaptic aberrations in principal neurons.

Authors - Naila Haq
  • KO Validated
humanIF

Dev Cell

The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.

Authors - Tomoharu Kanie
mouseIF

Autophagy

The ciliary protein RPGRIP1L governs autophagy independently of its proteasome-regulating function at the ciliary base in mouse embryonic fibroblasts.

Authors - Andreas Struchtrup
humanWB

EMBO Rep

Aurora Kinase A proximity map reveals centriolar satellites as regulators of its ciliary function.

Authors - Melis D Arslanhan
mouseWB

Elife

Islet vascularization is regulated by primary endothelial cilia via VEGF-A-dependent signaling.

Authors - Yan Xiong

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Christine (Verified Customer) (01-27-2023)

Detect 1 band above 47 kDa marker (expected size of BBS4 is 50-60 kDa), but also a much stronger band just below the 20 kDa marker

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: HEK293T

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