BBS10 Polyklonaler Antikörper

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• KD/KO Validated

BBS10 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12421-2-AP

Synonyme

C12orf58, Bardet-Biedl syndrome 10 protein, Bardet Biedl syndrome 10

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 12421-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12421-2-AP (BBS10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12421-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12421-2-AP (BBS10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12421-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12421-2-AP (BBS10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of hTERT-RPE1 cells using 12421-2-AP

  IF result (nuclear and the base of cilia stain) of anti-BBS10 (12421-2-AP; 1:50) with hTERT-RPE1 cell (PFA fixed) by Dr. Moshe Kim.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A549-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Produktinformation

12421-2-AP bindet in WB, IF/ICC, ELISA BBS10 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BBS10 fusion protein Ag3073
• Vollständiger Name: Bardet-Biedl syndrome 10
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 81 kDa
• GenBank-Zugangsnummer: BC013795
• Gene symbol: BBS10
• Gene ID (NCBI): 79738
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BBS10, also named as C12orf58, belongs to the TCP-1 chaperonin family. It is probable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, BBS10 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation. (PMID:20080638,19190184) Defects in BBS10 are the cause of Bardet-Biedl syndrome type 10 (BBS10).

Publikationen

Species	Application	Title
human	WB,IF	Proc Natl Acad Sci U S A; Transient ciliogenesis involving Bardet-Biedl syndrome proteins is a fundamental characteristic of adipogenic differentiation.; Authors - Marion Vincent V ; KD Validated
human,mouse	WB,IF	Proc Natl Acad Sci U S A; BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.; Authors - Seo Seongjin S
mouse	WB	Cilia; Comparing the Bbs10 complete knockout phenotype with a specific renal epithelial knockout one highlights the link between renal defects and systemic inactivation in mice.; Authors - Noëlle Cognard ; KO Validated