BAP1 Polyklonaler Antikörper

BAP1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10398-1-AP

Synonyme

BAP1, BRCA1 associated protein 1, Cerebral protein 6, HUCEP 13, hucep 6, KIAA0272, UCHL2

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Galerie der Validierungsdaten

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  WB analysis of A2780 using 10398-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10398-1-AP (BAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 10398-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10398-1-AP (BAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 10398-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10398-1-AP (BAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10398-1-AP

  IP result of anti-BAP1 (IP:10398-1-AP, 4ug; Detection:10398-1-AP 1:300) with HeLa cells lysate 3000ug.

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  IHC staining of human ovary tumor using 10398-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10398-1-AP (BAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10398-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10398-1-AP (BAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10398-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10398-1-AP (BAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10398-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10398-1-AP (BAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, humanes Plazenta-Gewebe, Maus-Eierstockgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

10398-1-AP bindet in WB, IP, IHC, ELISA BAP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BAP1 fusion protein Ag0597
• Vollständiger Name: BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase)
• Berechnetes Molekulargewicht: 81-91 kDa
• Beobachtetes Molekulargewicht: 91 kDa
• GenBank-Zugangsnummer: BC001596
• Gene symbol: BAP1
• Gene ID (NCBI): 8314
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BRCA1-associated protein-1 (BAP1),also named as KIAA0272, hucep-6, DKFZp686N04275, FLJ35406, FLJ37180, HUCEP-13, is a deubiquitinating enzyme whose function in the control of the cell cycle requires both its deubiquitinating activity and nuclear localization(PMID:22374320). It helps to control cell proliferation by regulating HCFC1 protein levels and by associating with genes involved in the G(1)-S transition)(PMID:19188440). Defects in BAP1 may be a cause of mesothelioma malignant (MESOM)(PMID:21642991) and tumor predisposition syndrome (TPDS)(PMID:21874003). The all BAP1s except those of C. quinquefasciatus have a molecular mass of more than 90 kDa(PMID:22374320).

Publikationen

Species	Application	Title
human	WB	Cancer Immunol Res; USP22 deubiquitinates CD274 to suppress anti-cancer immunity.; Authors - Xing Huang
human	WB	iScience; Centrosomal protein 120 promotes centrosome amplification and gastric cancer progression via USP54-mediated deubiquitination of PLK4; Authors - Chenggang Zhang
mouse	WB	Nature; Basis of the H2AK119 specificity of the Polycomb repressive deubiquitinase; Authors - Weiran Ge