BAF53B Polyklonaler Antikörper

BAF53B Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 27215-1-AP

Synonyme

actin like 6B, Actin like protein 6B, Actin related protein Baf53b, ACTL6, ACTL6B, ArpNalpha, BAF53B, BRG1 associated factor 53B

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 27215-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27215-1-AP (BAF53B antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 27215-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 27215-1-AP (BAF53B antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

27215-1-AP bindet in WB, ELISA BAF53B und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BAF53B fusion protein Ag25745
• Vollständiger Name: actin-like 6B
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47-53 kDa
• GenBank-Zugangsnummer: BC020944
• Gene symbol: ACTL6B
• Gene ID (NCBI): 51412
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAF53B, also named as ACTL6B, encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. BAF53b is unique among nucleosome remodeling complex subunits because it is neuron specific and is not found in any other nucleosome remodeling complex besides the nBAF complex(PMID: 23525042). BAF53B can be detected as about 47-53 kDa.

Publikationen

Species	Application	Title
human	WB	J Neurosci Res; An epilepsy-associated ACTL6B variant captures neuronal hyperexcitability in a human induced pluripotent stem cell model.; Authors - Lucie Y Ahn