• Überblick
  • Geprüfte Anwendungen
  • Produktinformation
  • Publikationen (33)
  • Protokolle
  • Rezensionen (1)
  • Featured Product
  • KD/KO Validated

BACE1 Polyklonaler Antikörper

BACE1 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte, Zebrafisch

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12807-1-AP

Synonyme

Beta-site amyloid precursor protein cleaving enzyme 1, Beta-secretase 1, Beta secretase 1, Aspartyl protease 2, ASP21



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaushirngewebe, HeLa-Zellen, humanes Hirngewebe, Maus-Pankreasgewebe, PC-12-Zellen, Rattenhirngewebe, Zebrafischgewebe
Erfolgreiche IPMaushirngewebe
Erfolgreiche Detektion in IHCMaushirngewebe, humanes Pankreaskarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PMaushirngewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:100-1:400
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

12807-1-AP bindet in WB, IHC, IF-P, IP, ELISA BACE1 und zeigt Reaktivität mit human, Maus, Ratten, Zebrafisch

Getestete Reaktivität human, Maus, Ratte, Zebrafisch
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen BACE1 fusion protein Ag3559
Vollständiger Name beta-site APP-cleaving enzyme 1
Berechnetes Molekulargewicht 501 aa, 56 kDa
Beobachtetes Molekulargewicht 70-75 kDa, 42-56 kDa
GenBank-ZugangsnummerBC036084
Gene symbol BACE1
Gene ID (NCBI) 23621
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

beta-secretase, encoded by BACE1(beta-stie amyloid beta A4 precursor protein-cleavage enzyem1) gene, is a neuron-specific membrane-associated protease that participate in the two step proteolytic processing of the APP. Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has a high expression in the brain and pancreas. This protein has 6 isoforms produced by alternative splicing. BACE1 can be detected with different molecular weight: 42-56kD (prozymogen and isoforms), 65-75 (glycosylated forms) and 160kDa (dimer) (PMID:22741101; 21795680).

Protokolle

Produktspezifische Protokolle
WB protocol for BACE1 antibody 12807-1-APProtokoll herunterladen
IHC protocol for BACE1 antibody 12807-1-APProtokoll herunterladen
IF protocol for BACE1 antibody 12807-1-APProtokoll herunterladen
IP protocol for BACE1 antibody 12807-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

  • Journal Impact Factor
  • Most recent
SpeciesApplicationTitle
humanWB

Small

Brain-Penetration and Neuron-Targeting DNA Nanoflowers Co-Delivering miR-124 and Rutin for Synergistic Therapy of Alzheimer's Disease.

Authors - Qin Ouyang
mouseWB

Redox Biol

Hyperoside alleviates toxicity of β-amyloid via endoplasmic reticulum-mitochondrial calcium signal transduction cascade in APP/PS1 double transgenic Alzheimer's disease mice

Authors - Lin Lin Song
mouseWB

Redox Biol

A potent phosphodiester Keap1-Nrf2 protein-protein interaction inhibitor as the efficient treatment of Alzheimer's disease

Authors - Yi Sun
mouseWB

Proc Natl Acad Sci U S A

Aβ-induced Golgi fragmentation in Alzheimer's disease enhances Aβ production.

Authors - Gunjan Joshi
human,mouseWB

Cell Rep

CIP2A Causes Tau/APP Phosphorylation, Synaptopathy, and Memory Deficits in Alzheimer's Disease.

Authors - Yang-Ping Shentu
mouseWB

Stem Cell Reports

BACE1 Deficiency Causes Abnormal Neuronal Clustering in the Dentate Gyrus.

Authors - Hailong Hou
  • KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Xiaoyu (Verified Customer) (02-07-2024)

Good signal.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: brain
BACE1 Antibody Western Blot validation (1:1000 dilution) in brain (Cat no:12807-1-AP)

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