B3GALT6 Polyklonaler Antikörper

B3GALT6 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55049-1-AP

Synonyme

B3GALT6, Beta 1,3 GalTase 6, Beta3Gal T6, beta3GalT6, GAG GalTII, Galactosyltransferase II

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 55049-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55049-1-AP (B3GALT6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 55049-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55049-1-AP (B3GALT6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 55049-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55049-1-AP (B3GALT6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 55049-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55049-1-AP (B3GALT6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 55049-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55049-1-AP (B3GALT6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 55049-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55049-1-AP (B3GALT6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 55049-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55049-1-AP (B3GALT6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 55049-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55049-1-AP (B3GALT6 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

55049-1-AP bindet in WB, IHC,ELISA B3GALT6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: UDP-Gal:betaGal beta 1,3-galactosyltransferase polypeptide 6
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: NM_080605
• Gene symbol: B3GALT6
• Gene ID (NCBI): 126792
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

B3GALT6 belongs to the glycosyltransferase 31 family. It transfers galactose from UDP-galactose to substrates with a terminal beta-linked galactose residue. B3GALT6 has a preference for galactose-beta-1,4-xylose that is found in the linker region of glycosaminoglycans, such as heparan sulfate and chondroitin sulfate. It has no activity towards substrates with terminal glucosamine or galactosamine residues. The antibody is specific to B3GALT6.