Annexin VI Monoklonaler Antikörper

Annexin VI Monoklonal Antikörper für FC, IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2H7G7

Kat-Nr. : 68086-1-Ig

Synonyme

67 kDa calelectrin, Annexin 6, annexin A6, Annexin VI, ANX6, ANXA6, Calphobindin II, CBP68, Chromobindin 20, CPB II, Lipocortin VI, p68, p70, Protein III

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Galerie der Validierungsdaten

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  WB analysis using 68086-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68086-1-Ig (Annexin VI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 68086-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68086-1-Ig (ANXA6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 68086-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Annexin VI antibody (68086-1-Ig, Clone: 2H7G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of K-562 using 68086-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Annexin VI (68086-1-Ig, Clone:2H7G7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, Hausschwein-Hirngewebe, HeLa-Zellen, humanes Plazenta-Gewebe, Kaninchenhirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68086-1-Ig bindet in WB, IF, FC, ELISA Annexin VI und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Annexin VI fusion protein Ag3263
• Vollständiger Name: annexin A6
• Berechnetes Molekulargewicht: 673 aa, 76 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC017046
• Gene symbol: ANXA6
• Gene ID (NCBI): 309
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.