Angiopoietin 1 Monoklonaler Antikörper

Angiopoietin 1 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

3C4A3

Kat-Nr. : 68618-1-Ig

Synonyme

ANGPT1, ANG-1, ANG1, ANG 1, AGPT

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  WB analysis using 68618-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68618-1-Ig (ANGPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HUVEC using 68618-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Angiopoietin 1 antibody (68618-1-Ig, Clone: 3C4A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, PNGase F treated A375 cells, non-treated U-87 MG cells, PNGase F treated U-87 MG cells, non-treated HuH-7 cells, PNGase F treated HuH-7 MG cells, non-treated bENd.3 cells, PNGase F treated bENd.3 cells
• Erfolgreiche Detektion in IF/ICC: HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

68618-1-Ig bindet in WB, IF/ICC, ELISA Angiopoietin 1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Angiopoietin 1 fusion protein Ag18829
• Vollständiger Name: angiopoietin 1
• Berechnetes Molekulargewicht: 498 aa, 58 kDa
• Beobachtetes Molekulargewicht: 70 kDa, 55 kDa
• GenBank-Zugangsnummer: BC152411
• Gene symbol: Angiopoietin 1
• Gene ID (NCBI): 284
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.

Publikationen

Species	Application	Title
mouse		Regen Biomater; Dandelion-shaped strontium-gallium microparticles for the hierarchical stimulation and comprehensive regulation of wound healing; Authors - Minrui Ji
human	WB	Stem Cell Res Ther; Lipid droplet-free nanovesicles extruded from stromal vascular fraction improve adipocyte regeneration in the centre of dermal graft; Authors - Yuyang Zeng
human	WB	Biol Reprod; Therapeutic targeting of the tryptophan-kynurenine Axis for HTR-8/SVneo trophoblast proliferation and migration in unexplained recurrent spontaneous abortion; Authors - Pingping Jin
human,mouse	WB,IF	Tissue Cell; Activation of the SIRT1/PGC-1α pathway by HNRNPD promotes vasculogenic mimicry in NSCLC; Authors - Xuefeng Zhang