Zinc Alpha 2 Glycoprotein Polyklonaler Antikörper

Zinc Alpha 2 Glycoprotein Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13399-1-AP

Synonyme

AZGP1, Zinc-alpha-2-glycoprotein, Zn alpha 2 GP, Zn alpha 2 glycoprotein, ZAG

---

Galerie der Validierungsdaten

• 

  WB analysis of human plasma using 13399-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 13399-1-AP (Zinc-alpha-2-glycoprotein antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13399-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13399-1-AP (Zinc-alpha-2-glycoprotein antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 13399-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13399-1-AP (Zinc Alpha 2 Glycoprotein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 13399-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13399-1-AP (Zinc Alpha 2 Glycoprotein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13399-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13399-1-AP (Zinc-alpha-2-glycoprotein antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 13399-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Zinc Alpha 2 Glycoprotein antibody (13399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of PC-3 using 13399-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using Zinc Alpha 2 Glycoprotein antibody (13399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 4 publications below

Produktinformation

13399-1-AP bindet in WB, IHC, IF/ICC, ELISA Zinc Alpha 2 Glycoprotein und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Zinc Alpha 2 Glycoprotein fusion protein Ag4232
• Vollständiger Name: alpha-2-glycoprotein 1, zinc-binding
• Berechnetes Molekulargewicht: 298 aa, 34 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC033830
• Gene symbol: Zinc Alpha 2 Glycoprotein
• Gene ID (NCBI): 563
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zinc-alpha-2-glycoprotein (AZGP1) is a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).

Publikationen

Species	Application	Title
human	IHC	Sci Adv; Mucus concentration-dependent biophysical abnormalities unify submucosal gland and superficial airway dysfunction in cystic fibrosis.; Authors - Takafumi Kato
human	IHC	Int J Cancer; Reduced AZGP1 expression is an independent predictor of early PSA recurrence and associated with ERG fusion positive and PTEN deleted prostate cancers.; Authors - Christoph Burdelski
human	IHC	J Transl Med; Decreased expression of zinc-alpha2-glycoprotein in hepatocellular carcinoma associates with poor prognosis.; Authors - Huang Yan Y
human	WB	Proteome Sci; Identification of low-abundance proteins via fractionation of the urine proteome with weak anion exchange chromatography.; Authors - Lu Chih-Ming CM
human	IHC	Obesity (Silver Spring); PMID: 24753506; Authors - Miroslav Balaz