ATRIP Polyklonaler Antikörper

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• KD/KO Validated

ATRIP Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11327-1-AP

Synonyme

AGS1, ATR interacting protein, ATRIP

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Galerie der Validierungsdaten

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  WB analysis using 11327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11327-1-AP (ATRIP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11327-1-AP

  WB result of ATRIP antibody (11327-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATRIP transfected HeLa cells.

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  IP experiment of HeLa using 11327-1-AP

  IP result of anti-ATRIP (IP:11327-1-AP, 4ug; Detection:11327-1-AP 1:5000) with HeLa cells lysate 1200 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MCF-7-Zellen, PC-3-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

11327-1-AP bindet in WB, IP, IHC, ELISA ATRIP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATRIP fusion protein Ag1869
• Vollständiger Name: ATR interacting protein
• Berechnetes Molekulargewicht: 791 aa, 86 kDa
• Beobachtetes Molekulargewicht: 86-90 kDa
• GenBank-Zugangsnummer: BC014153
• Gene symbol: ATRIP
• Gene ID (NCBI): 84126
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATRIP (ATR Interacting Protein) and ATR signalling complex is a central mediator of the replicative stress response (RSR),and mutations in both genes have been reported in Seckel syndrome.

Publikationen

Species	Application	Title
mouse	WB,IHC	Biomaterials; A fluorinated peptide with high serum- and lipid-tolerence for the delivery of siRNA drugs to treat obesity and metabolic dysfunction.; Authors - Jin Qiu ; KD Validated
human	WB	Front Pharmacol; Huangkui Capsule Ameliorates Renal Fibrosis in a Unilateral Ureteral Obstruction Mouse Model Through TRPC6 Dependent Signaling Pathways.; Authors - Li-Fei Gu
mouse	WB	Biochem J; Mutation of the RelA(p65) Thr505 phosphosite disrupts the DNA replication stress response leading to CHK1 inhibitor resistance; Authors - Jill E Hunter
mouse	WB	Biochem J; Regulation of CHK1 inhibitor resistance by a c-Rel and USP1 dependent pathway; Authors - Jill E Hunter