ATPIF1 Polyklonaler Antikörper

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• KD/KO Validated

ATPIF1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 12067-1-AP

Synonyme

IF(1), ATPIP, ATPI, ATPase inhibitory factor 1, ATP5IF1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 12067-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12067-1-AP (ATPIF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12067-1-AP

  IP result of anti-ATPIF1 (IP:12067-1-AP, 4ug; Detection:12067-1-AP 1:500) with HeLa cells lysate 3440ug.

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  IHC staining of human liver using 12067-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12067-1-AP (ATPIF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 12067-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12067-1-AP (ATPIF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12067-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATPIF1 antibody (12067-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 7 publications below

Produktinformation

12067-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ATPIF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATPIF1 fusion protein Ag2704
• Vollständiger Name: ATPase inhibitory factor 1
• Berechnetes Molekulargewicht: 106 aa, 12 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC009677
• Gene symbol: ATPIF1
• Gene ID (NCBI): 93974
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The H(+)-ATP synthase is a reversible engine of mitochondria that synthesizes or hydrolyzes ATP upon changes in cell physiology. ATP synthase dysfunction is involved in the onset and progression of diverse human pathologies. ATPIF1 gene encodes mitochondrial ATPase Inhibitory Factor 1 (IF1), also named ATPI, ATPIP or IP. Endogenous IF1 limits ATP depletion when the mitochondrial membrane potential falls below a threshold and the ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Mitochondrial content of IF1 controls the activity of oxidative phosphorylation mediating the shift of cancer cells to an enhanced aerobic glycolysis, thus supporting an oncogenic role of IF1 in cancer.

Publikationen

Species	Application	Title
human	IF	Nat Biotechnol; Magnify is a universal molecular anchoring strategy for expansion microscopy; Authors - Aleksandra Klimas
	IF	Br J Pharmacol; The compound BTB06584 is an IF1 -dependent selective inhibitor of the mitochondrial F1 Fo-ATPase.; Authors - Fabrice Ivanes
human	IF,WB	Cell Biosci; ATPIF1 maintains normal mitochondrial structure which is impaired by CCM3 deficiency in endothelial cells.; Authors - Kang Wang
	WB,IF	Lab Invest; ATP synthase inhibitory factor subunit 1 regulates islet β-cell function via repression of mitochondrial homeostasis; Authors - Kailiang Zhang ; KD Validated
	WB,IF	Lab Invest; ATP synthase inhibitory factor subunit 1 regulates islet β-cell function via repression of mitochondrial homeostasis.; Authors - Kailiang Zhang ; KD Validated
	IHC,IF	Front Physiol; F1F0-ATP Synthase Inhibitory Factor 1 in the Normal Pancreas and in Pancreatic Ductal Adenocarcinoma: Effects on Bioenergetics, Invasion and Proliferation.; Authors - Helen Tanton