ATP6V1A Polyklonaler Antikörper

ATP6V1A Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 51152-1-AP

Synonyme

ATP6A1, ATP6V1A, ATP6V1A1, HO68, V ATPase 69 kDa subunit, V ATPase subunit A, VA68, Vacuolar ATPase isoform VA68, Vma1, VPP2

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 51152-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51152-1-AP (ATP6V1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 51152-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 51152-1-AP (ATP6V1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 51152-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 51152-1-AP (ATP6V1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 51152-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 51152-1-AP (ATP6V1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 51152-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 51152-1-AP (ATP6V1A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 51152-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 51152-1-AP (ATP6V1A Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, humanes Hirngewebe, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

51152-1-AP bindet in WB, IHC, ELISA ATP6V1A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: NM_001690
• Gene symbol: ATP6V1A
• Gene ID (NCBI): 523
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.This antibody is specific to ATP6V1A.