SERCA3 Polyklonaler Antikörper

SERCA3 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 13619-1-AP

Synonyme

ATP2A3, Calcium pump 3, SERCA3, SR Ca(2+) ATPase 3

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Galerie der Validierungsdaten

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  WB analysis using 13619-1-AP

  Mouse heart lysates were subjected to SDS PAGE followed by western blot with 13619-1-AP (SERCA3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 13619-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13619-1-AP (SERCA3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 13619-1-AP

  IP result of anti-SERCA3 (IP:13619-1-AP, 4ug; Detection:13619-1-AP 1:500) with mouse skeletal muscle tissue lysate 3600ug.

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  IHC staining of human pancreas cancer using 13619-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13619-1-AP (SERCA3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 13619-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13619-1-AP (SERCA3 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, K-562-Zellen
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

13619-1-AP bindet in WB, IP, IHC, ELISA SERCA3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SERCA3 fusion protein Ag4527
• Vollständiger Name: ATPase, Ca++ transporting, ubiquitous
• Berechnetes Molekulargewicht: 1043 aa, 114 kDa
• Beobachtetes Molekulargewicht: 97-110 kDa
• GenBank-Zugangsnummer: BC035729
• Gene symbol: SERCA3
• Gene ID (NCBI): 489
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The SERCA, which pumps cytosolic Ca2+ into the ER, is an imperative maintainer of ER Ca2+ homeostasis. The mammalian SERCA family is comprised of three tissue-specific members, SERCA1-3. SERCA3 is present in endothelial and epithelial cells of various tissues. SERCA3 helps regulate intracellular Ca2+, which is critical for maintaining normal INS secretion and is closely linked to metabolism, neuronal plasticity, gene transcription, cell growth, differentiation, apoptosis, protein folding and carcinogenesis. Several isoforms of SERCA3 exist due to the alternative splicing.

Publikationen

Species	Application	Title
mouse	WB	Front Pharmacol; Astragaloside IV Attenuates Podocyte Apoptosis Mediated by Endoplasmic Reticulum Stress through Upregulating Sarco/Endoplasmic Reticulum Ca(2+)-ATPase 2 Expression in Diabetic Nephropathy.; Authors - Hengjiang Guo
human	WB	Acta Pharm Sin B; Highly expressed SERCA2 triggers tumor cell autophagy and is a druggable vulnerability in triple-negative breast cancer; Authors - Minmin Fan
mouse	IHC,WB	Nat Commun; Rescuing SERCA2 pump deficiency improves bone mechano-responsiveness in type 2 diabetes by shaping osteocyte calcium dynamics; Authors - Xi Shao
mouse	WB	Front Physiol; Proteomic analyses of urinary exosomes identify novel potential biomarkers for early diagnosis of sickle cell nephropathy, a sex-based study; Authors - Balamurugan Packialakshmi