ATP1A3 (middle) Polyklonaler Antikörper

ATP1A3 (middle) Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 28030-1-AP

Synonyme

ATP1A3, ATP1A3 (middle), DYT12, RDP, Sodium pump subunit alpha 3

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Galerie der Validierungsdaten

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  WB analysis using 28030-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28030-1-AP (ATP1A3 (middle) antibody) at dilution of 1:14000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 28030-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 28030-1-AP (ATP1A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 28030-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28030-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 28030-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28030-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 28030-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 28030-1-AP (ATP1A3 (middle) antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, SH-SY5Y-Zellen, SKOV-3-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

28030-1-AP bindet in WB, IHC, IF, ELISA ATP1A3 (middle) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP1A3 (middle) fusion protein Ag27705
• Vollständiger Name: ATPase, Na+/K+ transporting, alpha 3 polypeptide
• Berechnetes Molekulargewicht: 113 kDa
• Beobachtetes Molekulargewicht: 113 kDa
• GenBank-Zugangsnummer: BC015566
• Gene symbol: ATP1A3
• Gene ID (NCBI): 478
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP1A3 participates in the catalyticing hydrolysis of ATP and the exchanging of sodium and potassium ions across plasma membrane. The catalyticing activity mode is ATP + H2O + Na⁺(In) + K⁺(Out) = ADP + phosphate + Na⁺(Out) + K⁺(In). It has been published that the neurologic disorders rapid-onset dystonia-parkionsonism (RDP), alternating hemiplegia of childhood (ACH) and CAPOS syndrome (cerebellar ataxia, areflexia, pes cavus, optic atrophy and sensorineural hearing loss) are all related with the mutation of ATP1A3. There are other reports suggest that early life epilepsy and episodic apnea revealing are potentially associated with the mutation of ATP1A3 as a result of impairment of Na⁺/K⁺ homeostasis. This antibody is generated agaist the middle region (378-580aa) of ATP1A3 and detects 113 kDa protein in SDS-PAGE.(PMID: 30097153, 20301294, 29922587)

Publikationen

Species	Application	Title
human	WB, IF	Sci Adv; De novo ATP1A3 variants cause polymicrogyria.; Authors - Satoko Miyatake
human	WB	Nat Commun; Homophilic ATP1A1 binding induces activin A secretion to promote EMT of tumor cells and myofibroblast activation.; Authors - Yi-Ing Chen