ATP1A3 Polyklonaler Antikörper

ATP1A3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25727-1-AP

Synonyme

ATP1A3, DYT12, RDP, Sodium pump subunit alpha 3

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Galerie der Validierungsdaten

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  WB analysis using 25727-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25727-1-AP (ATP1A3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 25727-1-AP

  IP Result of anti-ATP1A3 (IP:25727-1-AP, 4ug; Detection:25727-1-AP 1:500) with rat brain tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 25727-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25727-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 25727-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25727-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 25727-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ATP1A3 antibody (25727-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 25727-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ATP1A3 antibody (25727-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche IP: Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25727-1-AP bindet in WB, IP, IHC, IF, ELISA ATP1A3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP1A3 fusion protein Ag22842
• Vollständiger Name: ATPase, Na+/K+ transporting, alpha 3 polypeptide
• Berechnetes Molekulargewicht: 1013 aa, 112 kDa
• Beobachtetes Molekulargewicht: 110-113 kDa
• GenBank-Zugangsnummer: BC015566
• Gene symbol: ATP1A3
• Gene ID (NCBI): 478
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP1A3 participates in the catalyticing hydrolysis of ATP and the exchanging of sodium and potassium ions across plasma membrane. The catalyticing activity mode is ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In). It has been published that the neurologic disorders rapid-onset dystonia-parkionsonism (RDP), alternating hemiplegia of childhood (ACH) and CAPOS syndrome (cerebellar ataxia, areflexia, pes cavus, optic atrophy and sensorineural hearing loss) are all related with the mutation of ATP1A3. There are other reports suggest that early life epilepsy and episodic apnea revealing are potentially associated with the mutation of ATP1A3 as a result of impairment of Na/K homeostasis. This antibody is generated against the C-terminal region (665-1013aa) of ATP1A3 and detects the band around 100-113 kDa in SDS-PAGE.(PMID: 30097153, 20301294, 29922587)