ATOX1 Polyklonaler Antikörper

ATOX1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 22641-1-AP

Synonyme

ATOX1, ATX1, Copper transport protein ATOX1, HAH1, Metal transport protein ATX1

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Galerie der Validierungsdaten

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  WB analysis using 22641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22641-1-AP (ATOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 22641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22641-1-AP (ATOX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 22641-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 22641-1-AP (ATOX1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 22641-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22641-1-AP (ATOX1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 22641-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22641-1-AP (ATOX1 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of PC-3 using 22641-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using 22641-1-AP (ATOX1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, A549-Zellen, HEK-293T-Zellen, HEK-293-Zellen, NCI-H1299-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

22641-1-AP bindet in WB, IHC, IF, ELISA ATOX1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATOX1 fusion protein Ag18460
• Vollständiger Name: ATX1 antioxidant protein 1 homolog (yeast)
• Berechnetes Molekulargewicht: 68 aa, 8 kDa
• Beobachtetes Molekulargewicht: 7 kDa
• GenBank-Zugangsnummer: BC112248
• Gene symbol: ATOX1
• Gene ID (NCBI): 475
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Antioxidant 1 (ATOX1) is a copper chaperone to regulate copper homeostasis in cell. In the cytosol, ATOX1 always binds to copper and transfer to ATPase proteins in the trans-Golgi network, thereby promoting copper supply to various copper-dependent oxidereductases matured within the secretory vesicles (PMID: 28294521). Also, the protein could protect cells against oxidative damage and the expression levels of ATOX1 may help to the inflammatory response and antioxidant defense,even to modulate response to the cancer (PMID: 27472369). ATOX1 plays an important role in the physiology of the human central nervous system ,and it's highly expressed in the cerebral cortex and hippocampus (PMID: 30545441). Either cytosol or nucleus location of ATOX1 has been reported in different literations, which may be associated with cell status (PMID: 24445997; 31317143).

Publikationen

Species	Application	Title
mouse	WB	iScience; Hepatic depletion of nucleolar protein mDEF causes excessive mitochondrial copper accumulation associated with p53 and NRF1 activation; Authors - Jinsong Wei