ATF4 Polyklonaler Antikörper

ATF4 Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF, FC (Intra)

Konjugation

FITC Fluorescent Dye

Kat-Nr. : FITC-10835

Synonyme

ATF4, CREB 2, CREB2, DNA binding protein TAXREB67, FITC-10835, TAXREB67, TXREB



Geprüfte Anwendungen

Erfolgreiche Detektion in IFHepG2-Zellen, mit Tunicamycin behandelte HeLa-Zellen
Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IFSee 1 publications below

Produktinformation

FITC-10835 bindet in IF, FC (Intra) ATF4 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ATF4 fusion protein Ag1279
Vollständiger Name activating transcription factor 4 (tax-responsive enhancer element B67)
Berechnetes Molekulargewicht 39 kDa
Beobachtetes Molekulargewicht45-50 kDa
GenBank-ZugangsnummerBC022088
Gene symbol ATF4
Gene ID (NCBI) 468
Konjugation FITC Fluorescent Dye
Excitation/Emission maxima wavelengths498 nm / 526 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation[PMID: 19016586]. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies[PMID: 19726872]. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia[PMID: 18163433]. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer. Endogenous ATF4 protein has a molecular mass of 50kd. [PMID: 17726049]. This antibody is a rabbit polyclonal antibody raised against full length human ATF4 antigen. The antibody recognizes the 38kd ATF4 protein and its phosphorylated forms (50kd). ATF4 can bind DNA as a homodimer and as a heterodimer. ATF4 is ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4, so the molecular weight of ATF4 may be 70 kDa.

Protokolle

Produktspezifische Protokolle
IF protocol for FITC ATF4 antibody FITC-10835Protokoll herunterladen
FC protocol for FITC ATF4 antibody FITC-10835Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIF

Cancer Cell Int

MiR-876-5p modulates head and neck squamous cell carcinoma metastasis and invasion by targeting vimentin.

Authors - Yibo Dong