ATF1 Polyklonaler Antikörper

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• KD/KO Validated

ATF1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 11946-1-AP

Synonyme

TREB36, Cyclic AMP-dependent transcription factor ATF-1, cAMP-dependent transcription factor ATF-1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 11946-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11946-1-AP (ATF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11946-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11946-1-AP (ATF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 11946-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11946-1-AP (ATF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11946-1-AP

  IP result of anti-ATF1 (IP:11946-1-AP, 4ug; Detection:11946-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IF Staining of HeLa using 11946-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11946-1-AP (ATF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, humanes Hirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 1 publications below

Produktinformation

11946-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ATF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATF1 fusion protein Ag2548
• Vollständiger Name: activating transcription factor 1
• Berechnetes Molekulargewicht: 271 aa, 29 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC029619
• Gene symbol: ATF1
• Gene ID (NCBI): 466
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TFs play a vital role in maintaining cell homeostasis by combining DNA regulatory sequences to regulate gene transcription rates. Activating transcription factor 1 (ATF1) is a member of the ATF/CREB family of transcription factors (TFs), specifically interacting with the consensus ATF/CRE site 'TGACGTCA'(PMID: 28032861). ATF1 transcriptionally regulates the expressions of target genes to mediate various cellular processes, and ATF1 effects the progression of tumors (including lung cancer, colorectal cancer,and nasopharyngeal carcinoma).

Publikationen

Species	Application	Title
human	WB	Am J Hum Genet; Systematic Functional Interrogation of Genes in GWAS Loci Identified ATF1 as a Key Driver in Colorectal Cancer Modulated by a Promoter-Enhancer Interaction.; Authors - Jianbo Tian ; KD Validated
mouse	WB	Mol Ther Nucleic Acids; Mbd2 deficiency alleviates retinal cell apoptosisvia the miR-345-5p/Atf1 axis in high glucoseinjury and streptozotocin-induced diabetic mice.; Authors - Yanni Ge
human	WB	Sci Signal; The death-inducing activity of RIPK1 is regulated by the pH environment.; Authors - Kenta Moriwaki
human	WB	Front Cell Dev Biol; CREB1 and ATF1 Negatively Regulate Glutathione Biosynthesis Sensitizing Cells to Oxidative Stress.; Authors - Lina Zhao ; KD Validated
human	WB	J Biol Chem; The mRNA levels of heat shock factor 1 are regulated by thermogenic signals via the cAMP-dependent transcription factor ATF3.; Authors - Narendra Verma
human	WB	Cells; Perilipin 5 Protects against Cellular Oxidative Stress by Enhancing Mitochondrial Function in HepG2 Cells.; Authors - Yanjie Tan